防治番茄灰霉病的枯草芽胞杆菌BAB-1粉尘剂研制  

A Preparation of Bacillus subtitles BAB-1 DPC against Tomato Gray Mold

作　　者：赵卫松[1] 鹿秀云[1] 郭庆港[1] 王培培[1] 商俊燕[1] 年冠臻 张晓云[1] 董丽红[1] 李社增[1] 马平[1] ZHAO Weisong;LU Xiuyun;GUO Qinggang;WANG Peipei;SHANG Junyan;NIAN Guanzhen;ZHANG Xiaoyun;DONG Lihong;LI Shezeng＊;MA Ping＊(IPM Center of Hebei Province/Key Laboratory of Integrated Pest Management on Crops in Northern Region of North China, Ministry of Agriculture/Institute of Plant Protection, Hebei Academy of Agricultural and Forestry Sciences, Baoding 071000, China)

机构地区：[1]河北省农林科学院植物保护研究所/河北省农业有害生物综合防治工程技术研究中心/农业部华北北部作物有害生物综合治理重点实验室,保定071000

出　　处：《中国生物防治学报》2018年第1期99-108,共10页Chinese Journal of Biological Control

基　　金：公益性行业(农业)科研专项(201303025);国家重点研发计划(2017YFD0201100);河北省财政专项(F17C10007);河北省人才培养工程

摘　　要：目前我国农药登记的微生物杀菌剂主要为水剂和可湿性粉剂两种剂型。为了克服常规喷雾导致温室大棚内湿度增加给防病带来的不利影响,本文以枯草芽胞杆菌BAB-1为有效成分开展了粉尘剂研究,通过对载体和助剂的筛选,明确了最佳的载体、助剂种类及其比例,最终确定100亿CFU/g枯草芽胞杆菌BAB-1粉尘剂的配方为:滑石粉20%、分散剂GY-D900 2%、菌株BAB-1原药补足至100%。该制剂具有很好的悬浮效果(30 min时悬浮率>50%)。室内盆栽试验表明,菌株BAB-1粉尘剂对番茄灰霉病防效达到57.14%~71.43%,对黄瓜灰霉病的保护作用优于治疗作用,施用1次的防病效果分别为48.80%和1.65%;温室大棚试验结果表明,该制剂在番茄大棚中施用3次后对灰霉病的防效达到79.04%,对照化学药剂嘧菌酯800倍水溶液的防效为82.55%。该研究结果为枯草芽胞杆菌BAB-1粉尘剂的进一步产业化开发和应用提供了科学依据。In present,wettable powder and aqueous solutions is the main types of microbial fungicide formulations in pesticide registration of China.Humidity was increased by the conventional spray method in greenhouse,in order to overcome the adverse effects for the disease prevention and control,Bacillus subtitles BAB-1 was used as active ingredient to develop the dust powder concentrate（DPC）.After carefully screening the suitable factors including carrier and additives,the optimal carrier,addit ive and their proportions were determined and employed for 1010 BAB-1 DPC formulation including talcum powder 20%,dispersing agent GY-D900 2%,up to 100% by BAB-1 technical material.This preparation of BAB-1 DPC had a excellent suspension rate more than 50% at 30 min.Pot experiment showed that the control efficacy of BAB-1 DPC was reached 57.14% to 71.43%,and the protective effect was superior to therapeutic action against tomato gray mold with control efficacy reached 48.80% and1.65% after applying once,respectively.In greenhouse experiment,the control efficacy of BAB-1 DPC and chemical fungicide azoxystrobin 800-fold aqueous solution after applying 3 t imes were 79.04% and 82.55%,respectively.The research results provided a scientific basis for further industrial development and application of Bacillus subtitles BAB-1 DPC.

关 键 词：枯草芽胞杆菌 相容性 粉尘剂 番茄灰霉病 防治效果 

分 类 号：S476[农业科学—农业昆虫与害虫防治]