基于ISSR、SCoT、SRAP标记的川续断属药用植物亲缘关系研究  

Analysis on genetic relationship of medicinal species of Dipsacus marked by ISSR,SCoT, and SRAP

作　　者：陈大霞[1,2,3,1] 张雪 王钰[1,2,3] 潘媛 李隆云[1,2,3] 易思荣 张泽[1] 

机构地区：[1]重庆市中药研究院,重庆400065 [2]重庆市中药良种选育与评价工程技术研究中心,重庆400065 [3]中国中医科学院中药资源中心重庆分中心,重庆400065 [4]重庆市药物种植研究所

出　　处：《中草药》2018年第4期903-909,共7页Chinese Traditional and Herbal Drugs

基　　金：国家“十一五”科技支撑计划(2006BA106A11.09);现代农业产业技术体系建设专项资金资助(CARS-21);重庆市特色效益农业中药材产业技术体系资助(2017[5]号)：重庆市“科技平台与基地建设”项目资助(cstc2014ptyjd10001);国家科技部科技人员服务企业行动项目(2009GJF10045)

摘　　要：目的研究9种川续断属药用植物的亲缘关系。方法采用ISSR、SCoT、SRAP 3种分子标记进行多态性检测,TREECONW分析软件计算遗传距离,UPGMA方法构建树状聚类图。结果 ISSR、SCoT、SRAP标记揭示的多态百分率差异不大,分别为90.4%、88.5%、88.2%,均表明供试材料的遗传多态性极为丰富;3种标记均揭示日本续断与大头续断之间遗传距离最大,表明二者亲缘关系最远;3种标记均将川续断属9种药用植物聚为3类,即大头续断与丽江续断、深紫续断与日本续断分别聚为一类,峨眉续断单独为一支,其余4个种聚为一类;ISSR标记和SRAP标记的聚类结果基本相同,仅是大理续断和川续断聚类先后略有差异。结论 3种标记的聚类结果与经典分类具有很好的吻合度,证实了分子标记技术的可靠性,可作为川续断属植物亲缘关系研究的有效方法之一。Objective To detect the genetic relationship among nine medicinal species of Dipsacus in China. Methods The polymorphic bands were detected by three labeling methods （ISSR, SCOT, and SRAP）. The genetic distances were calculated by TREECONW software and the systematic diagram ofgenetic relationship was clustered by UPGMA method. Results The percentage ofpolymorphism by ISSR, SCoT and SRAP markers showed little difference with value at 90.4%, 88.5%, and 88.2%, respectively, which indicated that the genetic polymorphism of the tested materials was very rich. The genetic distance between Dipsacus chinensis and D. japonicus was the largest, which indicated their genetic relationship was the most distant. Nine medicinal species of Dipsacus were all divided into three groups by three markers, that D. chinensi and D. lijiangensis, D. asperoides and D. japonicas were respectively clustered into one group, D. asperoides var. omiensis was alone clustered, the other four species were clustered into one group. The clustering results labeled by ISSR and SRAP were basically the same with a slight difference between D. daliensis and D. asperoides. Conclusion The clustering results bythere markers was between well consistent with the classical classification, which confirmed that molecular markers can be used as one of the effective methods to reveal the genetic relationship among medicinal species of Dipsacus.

关 键 词：川续断属 ISSR SCoT SRAP 亲缘关系 

分 类 号：R282.12[医药卫生—中药学]