ANGPTL2基因单核苷酸多态性与梗死相关动脉自发性再通的关系  

Association of single nucleotide polymorphism(SNPs) of ANGPTL2 gene with infarct-related artery spontaneous reperfusion

作　　者：张明亮[1] 杨文龙[2] 周旋 孙垭娉 张俊峰[2] 

机构地区：[1]蚌埠医学院,安徽蚌埠2330302 [2]上海交通大学医学院附属第九人民医院心内科,上海201999

出　　处：《心脏杂志》2018年第1期65-69,共5页Chinese Heart Journal

基　　金：国家自然科学基金项目资助(81670316);上海市自然科学基金项目资助(16ZR1419900)

摘　　要：目的研究血管生成素样蛋白(angiopoietin-like proteins,ANGPTL)2基因单核苷酸多态性(SNP)与梗死相关动脉自发性再通(SR)之间的相关性。方法提取36例SR患者和62例非自发性再通(NSR)患者的外周血DNA,采用荧光定量PCR对ANGPTL2基因的3个内含子区SNP位点[rs999092(A>G),rs11792699(A>G)和rs13298677(A>G)]进行扩增,然后进行sanger测序,结果与UCSC基因库中的标准序列进行比较,分析两组在各个SNP位点上基因型和等位基因的突变频率有无显著差异。结果 SR组患者尿酸、WBC和平均血小板体积(MPV)较NSR组低,3个SNP位点的基因型和等位基因突变频率,在两组间的差异无统计学意义,多因素logistic回归分析显示,尿酸和MPV是SR发生的独立预测因子。结论 ANGPTL2基因SNPs位点的突变频率差异与梗死动脉自发性再通无相关性。AIM To investigate the association of single nucleotide polymorphism of ANGPTL2 gene with infarct-related artery spontaneous reperfusion in patients with ST-segment elevation myocardial infarction （STEMI）. METHODS Genomic DNA was extracted from the peripheral blood of 36 cases of spontaneous reperfusion （SR） patients and 62 cases of non-spontaneous reperfusion （NSR） patients. Three SNPs （rs999092 （A〉G）, rs11792699 （A〉G） and rs13298677 （A〉G） of Angptl2 gene were amplified with fluorescence quantitative PCR and were detected by Sanger sequencing. The results were compared with the standard sequence of UCSC gene library and the differences of SNP locus genotype and allele frequencies in the two groups were analyzed. RESULTS Uric acid, white blood count, and mean platelet volume （MPV） in the SR group were lower than those in the NSR group. Logistic regression analysis showed that uric acid and MPV were independent predictors of SR and were negatively correlated with the occurrence of SR. No significant difference was found in the genotype and allele frequencies in 3 SNPs between the two groups. CONCLUSION Single nucleotide polymorphism of ANGPTL2 gene is not the cause of differential expression of ANGPTL2 in platelets between SR and NSR groups and is not correlated with spontaneous recanalization of infarct artery.

关 键 词：急性ST段抬高型心肌梗死 自发性再通 血管生成素样蛋白2 单核苷酸多态性 

分 类 号：R541.4[医药卫生—心血管疾病]