三种不同人群HPV检测DNA分型结果及其临床意义探讨  

Investigation of the clinical significance for HPV detection of DNA typing results in three different populations

作　　者：唐小勇[1] 钟万芬[1] 孙凯[1] 江华[1] 邢飞[2] 

机构地区：[1]襄阳市中心医院(湖北文理学院附属医院)检验科,湖北襄阳441021 [2]襄阳市中心医院(湖北文理学院附属医院)皮肤科,湖北襄阳441021

出　　处：《中国性科学》2018年第1期44-47,共4页Chinese Journal of Human Sexuality

基　　金：湖北省自然科学基金项目(2012FFA071)

摘　　要：目的:探究DNA分型检测对三种不同人群的检测的临床意义。方法:随机选择2015年7月至2016年7月间我院皮肤科门诊诊断的109例尖锐湿疣患者为观察组,选择同时期入院检查的117例无症状的高危自检者为B对照组,选取112例定期健康检查、孕前及婚前正常体检者为A对照组。用镊子取观察组尖锐湿疣患者的可见疣体组织0.10~0.50 mm^3;A和B对照组无疣体的参试者,用专用的HPV采样刷在女性外阴或宫颈脱落细胞,男性在冠状沟或尿道口进行采样。按照实验室指标对参试者进行检测后,并结合医院自行设计的有关尖锐湿疣的规范准则,详细记录参试者的各项测量指标,最后对收集数据进行统计学分析。结果:经过检查,观察组患者的阳性检出率为93.58%,B对照组的阳性检出率为23.08%,A对照组的阳性检出率为4.46%,A对照组的阳性检出率明显低于B对照组和观察组,且B对照组明显低于观察组,差异有统计学意义(P<0.05)。参试者的HPV-DNA分型以感染单型6/11型为主,其次是16/18型和6/11+16/18混合型。其中单型6/11型明显高于16/18型和6/11+16/18混合型,差异对比有统计学意义(P<0.05)。109例尖锐湿疣患者阳性例数为102例,其中单一感染患者为81(74.31%),多重亚型感染为21(19.27%),其中低危型是单一感染的主要类型,高危型是多重感染的主要类型。结论:尖锐湿疣患者、无症状的高危自检者及正常人的分型检测中以6/11型HPV-DNA为主,高危人群诊断阳性率有明显差异,临床上应加强对此类高危人群的HPV-DNA的分型检测,以便尽早确诊及治疗。Objectives： To explore the DNA typing results of three different populations and their clinical significance in HPV. Methods： 109 patients with condyloma acuminatum（ CA） in our hospital from July 2015 to July 2016 were randomly selected as the observation group; 117 high-risk asymptomatic patients were as control group B; 112 regular health persons receiving health examination were as control group A. The CA tissue 0. 10 ~0. 50 mm3 visible warts were collected with tweezers in observation group; there was no wart in control group A and B. Female genital or cervical cell,and coronal or male urethra cell was collected with special HPV sampling. According to the laboratory index to detect the participants,and combined with the relevant hospital of CA norms designed,measured indicators,the collected data were statistically analyzed. Results： After examination,the positive rate in observation group was 93. 58%,the positive rate in control group B was 23. 08% and it was 4. 46% in control group A. The positive rate of control group A was significantly lower than these of control group B and observation group,and the positive rate of control group B was significantly lower than that of observation group. The differences were statistically significant（ P〈0. 05）. The single type 6/11 type was significantly higher than that of type16/18 and 6/11 ＋ 16/18 mixed type（ P〈0. 05）. There were 102 positive cases among 109 cases of CA. There were 81 single infection patients（ 74. 31%） and 21 multiple subtypes of infection patients（ 19. 27%）. The lowrisk type was the main type of single infection and high-risk type was the main type of multiple infection.Conclusion： The 6/11 type HPV-DNA is the main type among patients with CA,high-risk type asymptomatic patients and normal human. Typing detection of such high-risk HPV-DNA should be strengthened for early diagnosis and treatment.

关 键 词：HPV检测 三种不同人群 DNA分型 临床意义 

分 类 号：R752[医药卫生—皮肤病学与性病学]