组织纤溶酶原激活物的分离纯化  

PURIFICATION OF TISSUE PLASMINOGEN ACTIVATOR FROM PORCINE HEART

作　　者：周克元[1] 凌光鑫[1] 刘国勋[1] 尹小川[2] 曾令均[2] 邓漪平[2] 

机构地区：[1]湛江医学院生化教研室,广东624023 [2]中山医科大学组胚教研室,广州510060

出　　处：《中国医药工业杂志》1991年第12期529-533,共5页Chinese Journal of Pharmaceuticals

基　　金：卫生部(75-62-02-11)项目

摘　　要：用亲和层析法从猪心组织分离纯化纤溶酶原激活物(tissue type plasminogen activator,t-PA),以纤维蛋白-sepharose-4B 和 lys-sepharose-4B 两种亲和胶,从5 kg 新鲜猪心组织中分离出 t-PA1.46mg,比活性为235000 IU/mg,得率为34%,SDS 聚丙烯酰胺凝胶电泳测定为一条带,分子量为68000。Tissue type plasminogen activator was isolated and purifiedfrom porcine heart by affinity adsorption chromatography with fibrin-sepharose-4B and lys-sepharose-4B.One point forty six mg t-PA was obtained from 5kgheart tissue.The specific activity was 235000 IU/mg,and the yield was 34%,with a molecular weight of 68000 as measured by SDS-polyacrylamide gel elec-trophoresis.

关 键 词：纤溶酶 猪心 纯化 分离 

分 类 号：TQ464.52[化学工程—制药化工]