南方菜豆花叶病毒RT-RPA检测方法的建立  

One-step Reverse Transcription Recombinase Polymerase Amplification (RT-RPA) for the detection of Southern bean mosaic virus

作　　者：魏霜[1,2] 袁俊杰 李桂芬[1] 乾义柯 魏梅生[1] 冯黎霞[2] 胡学难[2] 何日荣[2] 武目涛[2] 张永江[1] 

机构地区：[1]中国检验检疫科学研究院,北京100176 [2]广东出入境检验检疫局检验检疫技术中心 [3]湛江出入境检验检疫局 [4]伊犁出入境检验检疫局

出　　处：《植物检疫》2018年第1期50-53,共4页Plant Quarantine

基　　金：广东出入境检验检疫局科技计划项目(2017GDK76);国家重点研发计划(2016YFF0203203);国家质量监督检验检疫总局科技计划项目(2017IK163)

摘　　要：本研究针对南方菜豆花叶病毒(Southern bean mosaic virus,SBMV)保守序列设计了特异性PRA引物,建立了快速灵敏的一步法逆转录重组酶聚合酶扩增检测技术(RT-RPA),采用SBMV,BPMV,SMV,Ar MV和TRSV共5种病毒评价该方法的特异性,并对其灵敏度进行评价。结果显示,建立的RT-RPA方法特异性强;灵敏度达到0.1 ng。本研究建立的RT-RPA方法检测SBMV特异性强、灵敏度高,无需特殊的仪器设备,适合实验室或者现场快速检测。A one-step reverse transcription recombinase polymerase amplification（RT-RPA） assay for the detection of Southern bean mosaic virus（SBMV） has been developed. Species-specific RPA primers were designed based on gene of SBMV. The specificity of this assay has been tested by SBMV,BPMV,SMV,Ar MV and TRSV. The results show that the RT-RPA assay was of high specificity for the detection of SBMV. The sensitivity of RT-RPA assay was 0.1 ng. In conclusion,the RT-RPA assay is high specific,sensitive and reliable,and will be an effective tool for the detection of SBMV.

关 键 词：南方菜豆花叶病毒 RPA 检测 

分 类 号：S41-31[农业科学—植物保护]