猪链球菌LuxS/AI-2型密度感应系统研究进展  

LuxS/AI-2 System of Streptococcus suis

作　　者：王瑜欣 汪洋[1] 易力 陆承平 

机构地区：[1]河南科技大学动物科技学院,洛阳471003 [2]洛阳师范学院生命科学学院,洛阳471022 [3]南京农业大学动物医学院农业部动物细菌学重点实验室,南京210095

出　　处：《畜牧兽医学报》2018年第2期223-230,共8页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家自然科学基金(31772761;31540095;31201910);河南省高校创新人才基金(14HASTIT024);河南省自然科学基金(162300410067)

摘　　要：密度感应(quorum sensing,QS)系统在细菌中普遍存在,能够通过一种或多种信号分子来影响多个基因的转录表达,从而协调多种细菌病原体的群体反应。该系统存在多种类型,其中LuxS/AI-2型是研究最热的系统之一,luxS作为一个高度保守的基因,参与调控多种基因的表达,从而调节细菌的生长和毒力等多种生命活动,使其适应不同的环境。LuxS蛋白分解形成同型半胱氨酸和4,5-二羟基-2,3-戊二酮(4,5-dihydroxy-2,3-pentanedione,DPD),DPD通过自环化形成自诱导分子autoinducer 2(AI-2)。猪链球菌(Streptococcus suis,SS)作为一种重要的人兽共患病原菌,研究其LuxS/AI-2型密度感应系统,利用LuxS/AI-2系统对猪链球菌病进行预防和治疗,具有重要意义。本文着重从SS的QS系统一般机制以及LuxS/AI-2系统的发现、结构、代谢作用、表达调控等方面对该菌的相关研究进行了综述。Quorum sensing（QS）system is a microbial cell-to-cell communication process that can influence the transcriptional expression of multiple genes by one or more signaling molecules to coordinate the population response of multiple bacterial pathogens.It relies on the production and detection of chemical signals called autoinducers to monitor cell density and species complexity in the population.The system includes a variety of types,which LuxS/AI-2 type is one of the hottest types,luxSas a highly conserved gene,involved in the regulation of a variety of gene expression,thereby regulating the growth and virulence of bacteria and other life activities,to adapt to different environments.LuxS protein cleaves S-ribosylhomocysteine to form homocysteine and 4,5-dihydroxy-2,3-pentanedione（DPD）which is then spontaneously cyclized to form AI-2.Streptococcus suis（SS）is a major swine pathogen and an emerging zoonotic agent of human meningitis and streptococcal toxic shock-like syndrome.It is of great significance to study the SS LuxS/AI-2 system and uses LuxS/AI-2 system to prevent and treat SS disease.This review focuses on the general mechanism of QS system in SS,the discovery,structure,metabolism,expression and regulation of LuxS/AI-2.

关 键 词：密度感应系统 LuxS/AI-2系统 猪链球菌 调控 

分 类 号：S852.611[农业科学—基础兽医学]