白魔芋组培技术研究  

Study on the Tissue Culture Technology of A.ablus liu et Chen

作　　者：何翠[1] 曾旭[1] 熊红兵[1] 罗玉华[1] 周文兵[1] 

机构地区：[1]宜宾市农业科学院,四川宜宾644000

出　　处：《现代农业科技》2018年第4期71-72,共2页Modern Agricultural Science and Technology

摘　　要：以白魔芋球茎为外植体,研究白魔芋组培技术。结果表明,75%酒精30 s+0.1%升汞15 min消毒效果较好,污染率较低,为11.11%;6-BA 1 mg/L+TDZ 0.2 mg/L+NAA 1 mg/L愈伤组织诱导效果较好,诱导率达到73.33%,且愈伤组织质量紧密;6-BA 1.5 mg/L+NAA 0.05 mg/L诱导芽过程中,出芽时间较早,分化率最高,达到91.11%,且在芽生长过程中同时生长出根,比较适合芽诱导。Taking the corm ofA.ablus liu et Chen as test material,tissue culture technology was studied.The results showed that disinfection effect of 75% alcohol 30 s＋0.1% HgC1 15 rain was better,pollution rate was 11.11% ;The induction effect of 6-BA 1 mg/L＋TDZ 2 mg/L＋NAA 1 mg/L was better ,and the induction rate reached 73.33% ,and the tissue quality was close.In the medium of 6-BA 1.5 mg/L＋NAA 0.05 mg/L ,the buds were induced to sprout earlier, with the highest differentiation rate, up to 91.11%, and in the process of bud growth, the roots grew, which was suitable for the bud induction.

关 键 词：白魔芋 组培技术 芽诱导 

分 类 号：S632.9[农业科学—蔬菜学]