益气养阴清热化瘀方对免疫性血小板减少症小鼠Notch信号通路的影响  

Effect of Yiqi Yangyin Qingre Huayu Recipe on Notch Signaling Pathway in Mice with Immune Thrombocytopenia

作　　者：谢东杰[1] 王爱迪[1] 王伟涛[2] 张乐[1] 张珊 刘宝山[1] 

机构地区：[1]天津医科大学总医院,天津300052 [2]天津中医药大学第一附属医院,天津300193

出　　处：《中国中医基础医学杂志》2018年第1期38-41,共4页JOURNAL OF BASIC CHINESE MEDICINE

基　　金：国家自然科学基金资助项目(81373847)-从Th17/Treg失衡和Notch通路探讨益气养阴清热化瘀方调控ITP血小板破坏及生成的机制

摘　　要：目的:明确益气养阴清热化瘀方对ITP的治疗作用并观察该方对Notch信号分子的影响,探讨其发挥效应的潜在机制。方法:60只小鼠按随机数字表法分为正常组10只、ITP模型组50只,于模型组小鼠腹腔每日注射含2μg大鼠抗小鼠CD41单克隆抗体(MWReg30单抗)的磷酸盐缓冲液200μL,造成持续的血小板减少症,建立ITP小鼠模型。第8天进行模型评估后,将模型组随机分为不同的治疗组进行药物干预2周,治疗结束后测量各组血小板计数;采用RT-PCR、Western blot法分别测量各组骨髓单个核细胞(BMMNC)Notch受体Notch1-4和Notch配体Jagged1,Jagged2基因和蛋白的表达。结果:与模型组比较,中药各剂量组均能显著升高血小板水平,明显降低Notch(1-4)、Jagged1、2基因和蛋白的表达。结论:益气养阴清热化瘀方能够通过下调Notch信号的表达水平对ITP的病理环节产生影响,从而发挥升板作用,达到治疗ITP的目的。Objective： To clarify the effect of Yiqi Yangyin Qingre Huayu Decoction for ITP and observe the influence for Notch molecular,exploring the potential mechanism for the treatment of ITP. Methods： 60 mice randomly divided into normal group（ n = 10） and ITP model group（ n = 50）,BALB/C mice were rendered thrombocytopenia by daily intraperitoneal injection of 2 μg rat anti-mouse CD41 monoclonal antibodies（ MWReg30） in 200 μL phosphate-buffered saline（ PBS） in model group. On the 8 th day,After the evaluation,the ITP model mice were divided into different drug intervention groups which was performed with different drug interventions for 2 weeks,the platelet count was observed after treatment and then the gene and protein expression levels of Notch1-4 and Jagged1-2 in the bone marrow mononuclear cells（ BMMNC） were measured by RT-PCR and Western blot respectively. Results： Each dose of Chinese medicine treatment group can significantly increase the platelet compared to model group and reduce notably Notch（ 1-4）,Jagged1,2 gene and protein expression levels. Conclusion： Yiqi Yangyin Qingre Huayu Decoction plays a role in elevating platelet level by reducing the expression of Notch signaling to influence the pathology progress of ITP and achieves eventually the purpose of treatment of ITP.

关 键 词：益气养阴清热化瘀方 免疫性血小板减少症 NOTCH信号通路 

分 类 号：R285.5[医药卫生—中药学]