吡咯喹啉醌二钠对产蛋鸡生产性能、抗氧化状态和血浆生化指标的影响  

Effects of Pyrroloquinoline Quinone Disodium on Performance,Antioxidant Status and Plasma Biochemical Parameters of Laying Hens

作　　者：王晶[1] 杨林林 张海军[1] 武书庚[1] 齐广海[1] 

机构地区：[1]中国农业科学院饲料研究所,农业部饲料生物技术重点开放实验室,生物饲料开发国家工程研究中心,北京100081

出　　处：《动物营养学报》2018年第2期667-677,共11页CHINESE JOURNAL OF ANIMAL NUTRITION

基　　金：国家蛋鸡产业技术体系项目(CARS-41-K13);国家自然科学基金项目(31172212)

摘　　要：本试验通过研究不同添加量的吡咯喹啉醌二钠(PQQ·Na_2)对产蛋鸡生产性能、抗氧化状态和血浆生化指标的影响,旨在评价PQQ·Na_2在产蛋鸡上的生物学安全性。试验选用540只25周龄的健康海兰灰蛋鸡,随机分为5组,每组设6个重复,每个重复18只。5组产蛋鸡分别饲喂添加0(对照)、0.04、0.08、0.12 mg/kg PQQ·Na_2及200 mg/kg维生素E的5种试验饲粮。预试期1周,正试期24周。结果表明:1)与对照组相比,饲粮添加0.04~0.12 mg/kg PQQ·Na_2对产蛋鸡的生产性能未产生显著影响(P>0.05);2)试验第24周,与对照组相比,饲粮添加0.04~0.12 mg/kg PQQ·Na_2显著提高了鸡蛋的哈氏单位(P<0.05),且添加0.08 mg/kg PQQ·Na_2还显著提高了蛋壳厚度(P<0.05);3)与对照组相比,饲粮添加0.08和0.12 mg/kg PQQ·Na_2显著提高了血浆谷胱甘肽过氧化物酶(第12周)和总超氧化物歧化酶活性(第2周、第24周)(P<0.05);4)与对照组相比,饲粮添加0.08 mg/kg PQQ·Na_2显著降低了心脏羰基含量(P<0.05),显著提高了肝脏总抗氧化能力(P<0.05),且添加0.12 mg/kg PQQ·Na_2还显著降低了肝脏丙二醛含量(P<0.05)。上述试验结果提示,饲粮添加0.08~0.12 mg/kg PQQ·Na_2对产蛋鸡的生产性能无不良影响,且可改善机体抗氧化功能,综合考虑得出产蛋鸡饲粮中PQQ·Na_2的适宜添加量为0.08 mg/kg。The current study aimed to evaluate the biological safety of pyrroloquinoline quinone disodium （ PQQ ·Na2 ） for laying hens and investigated the effects of dietary PQQ · Na2 suplemental levels on perform- ance, antioxidant status and plasma biochemical parameters. Five hundred and forty 25-week-old Hyline Grey laying hens were randomly divided into 5 groups with each group consisted of 6 replicates of 18 birds each. The laying hens in the 5 groups were fed 5 experimental diets with 0 （control）, 0.04, 0.08, 0.12 mg/kg PQQ ·Naz and 200 mg/kg vitamin E, respectively. All hens were fed the diets for a 1-week adaptation fol- lowed by a 24-week trial period. The result showed as follows： 1 ） diet supplemented with 0.04 to 0.12 mg/kg PQQ · Na2 did not affect the performance of laying hens （P〉0.05）. 2） Compared with the control group, the Haugh unit was significantly increased by diet supplemented with 0.04 to 0.12 mg/kg PQQ ~ Na2 （P〈0.05）, and the eggshell thickness was significantly increased by diet supplemented with 0.08 mg/kg PQQ ~ Na2 at the week 24 of trial （P〈0.05）. 3） Compared with the control group, the activities of plasma glutathion peroxidase （GSH-Px） （ week 12 ） and total superoxide dismutase （T-SOD） （ week 2 and 24 ） were significantly increased by diet supplemented with 0.08 and 0.12 mg/kg PQQ · Na2 （P〈0.05）. 4） Compared with the control group, diet supplemented with PQQ· Na2 at 0.08 mg/kg significantly decreased the content of heart carbonyl group and significantly increased the hepatic total antioxidant capacity （T-AOC） （P〈0.05）, and diet supplemented with 0.12 mg/kg PQQ · Na2 significantly decreased the hepatic malondialdehyde （MDA） content （P〈0.05）. Above results indicate that the supplementation of PQQ· Na2 at 0.08 to 0.12 mg/kg in laying hens＇ diet do not have negative effects, but can enhance the laying hens＇ antioxidant function. The suitable supplemental lev- el of PQQ · Na2 in laying hens＇ diet is 0.08 m

关 键 词：吡咯喹啉醌二钠 产蛋鸡 抗氧化 血浆生化指标 蛋品质 

分 类 号：S816.7[农业科学—饲料科学]