ABA及氟啶酮调控下冬小麦分蘖节抗寒相关蛋白的鉴定与分析  

Identification and Analysis on Cold-Resistant Relative Proteins Regulated by ABA and Fluridone in Winter Wheat(Triticum aestivum L.)

作　　者：杨宁[1] 包雨卓 赵浡彤 吕岩[1] 彭瞰看 田宇[1] 徐庆华[1] 于晶[1] 王军虹[1] 苍晶[1] 

机构地区：[1]东北农业大学生命科学学院,黑龙江哈尔滨150030 [2]广东白云学院,广东广州510450

出　　处：《麦类作物学报》2018年第2期213-220,共8页Journal of Triticeae Crops

基　　金：国家自然科学基金项目(31471423);国家自然科学人才培养基金科研训练项目(J1210069);黑龙江省自然科学基金项目(C201418)

摘　　要：为了研究ABA调控下冬小麦在蛋白质水平上的抗寒分子机制,以强抗寒冬小麦品种东农冬麦1号为材料,在外源ABA及氟啶酮处理后,于大田自然降温至4℃和-25℃时取分蘖节进行蛋白质双向电泳分析。对各处理组的双向电泳结果进行软件分析可知,4℃下得到可匹配的蛋白点587个,-25℃下得到可匹配蛋白点394个。筛选后选择差异显著的24个蛋白点进行质谱分析,经鉴定这些蛋白包括逆境蛋白(WCI16、LTR-Rbps、CORs)、代谢相关蛋白(PGAM、PGK、26sRP、ATPase-β、GST1、GST、GSTF5)、转录翻译相关蛋白(BTFs、eIF4A、eIF5A)和未知蛋白。In order to study the cold-resistant molecular mechanism in winter wheat which is on protein level and is regulated by ABA, the test material is dongnongdongmai 1 which is a strong cold-resistant wheat variety. After treated by exogenous ABA and Fluridone, tillering nodes in field were collected respectively at 4 ℃ and --25 ℃. A lot of differentially expressing proteins were analysed by two-di- mensional gel electrophoresis analysis. The results showed that： there were 587 matching protein spots at --4 ℃ and 394 matching protein spots at --25 ℃; after screening from above, 24 protein spots were identified by protein mass spectrometry; differentially expressing proteins are stress pro- teins（WCI16, LTR-Rbps, CORs）, metabolic proteins（PGAM, PGK, 26sRP, ATPase-[3, GST1, GST, GSTF5）, transcription and translation related proteins（BTFs, eIF4A, eIF5A） and unknown proteins.

关 键 词：东农冬麦1号 双向电泳 ABA 氟啶酮 抗寒蛋白 

分 类 号：S512.1[农业科学—作物学]