8周游泳运动对2型糖尿病大鼠心肌间质纤维化的影响  

The effect of 8 weeks swimming training on myocardial interstitial fibrosis in type 2 diabetic rats

作　　者：上官若男[1,2] 焦艺科 尚画雨 苏全生[4] 

机构地区：[1]北京体育大学运动人体科学学院,北京100084 [2]成都大学体育学院 [3]北京体育大学运动康复系 [4]成都体育学院

出　　处：《中国康复医学杂志》2018年第2期158-164,共7页Chinese Journal of Rehabilitation Medicine

摘　　要：目的:观察8周游泳运动对2型糖尿病大鼠心肌间质纤维化的影响。方法:健康雄性8周龄SPF级Wistar大鼠45只,随机抽取32只在高糖高脂饲料喂养7周的基础上腹腔注射小剂量STZ缓冲液,建立2型糖尿病大鼠模型。将正常大鼠和造模成功的大鼠分为4组:空白对照组(C组)、单纯运动组(CE组)、糖尿病对照组(DM组)、糖尿病运动组(DME组)。运动组采用改进的Ploug训练方案,60min/d,每周训练6d,共训练8周。运动8周末尾静脉取血测定空腹血糖(FBG),眶后取血测定空腹血清胰岛素(FINS),并计算胰岛素抵抗指数(HOMA-IR),HE染色和Masson染色观察心肌形态学变化,ELISA法测定心肌组织COLⅠ和COLⅢ含量。结果:(1)8周运动干预后,DM组和DME组FBG非常显著高于C组和CE组,FINS水平显著低于C组和CE组,HOMA-IR指数DM组非常显著高于C组和CE组、DME组显著高于C组和CE组;DME组与DM组相比,FBG和HOMA-IR指数显著降低,FINS水平显著升高;DM组和DME组心脏相对重量(HW/BW)显著高于C组;DME组与DM组、CE组与C组HW/BW值无显著性差异。(2)DM组大鼠心肌病理改变可见心肌纤维断裂、坏死、严重增生,心肌细胞排列紊乱,水肿明显,变性严重,间质充血;DME组心肌结构改变有所减轻,C组和CE组呈现正常的心肌细胞形态。(3)心肌组织COLⅠ含量DM组显著高于DME组、C组和CE组;DME组、C组和CE组之间无显著性差异;心肌组织COLⅢ含量各组差异均无显著性。结论:8周游泳运动明显改善2型糖尿病大鼠心肌间质纤维化程度,对大鼠心肌损伤具有保护作用。Objective： To observe the effect of 8 weeks swimming training on the myocardial fibrosis in Type II diabetic rats. Method： Totally 45 healthy male Wistar rats aged 8 weeks were employed feeding with high-fat diet for 7 weeks, and intraperitoneal injection of low-dose STZ buffer once were used to establish the model of Type 2 diabetes. The successful normal and model rats were divided into four groups： normal control group （C group,6）,normal exercise group （CE group,5）,diabetic group （DM group,7）,diabetic exercise group （DME group,6）. The exercise group was assigned to the Ploug training protocol, 6 days/week, 60min/day, 8 weeks in total. After 8 weeks, blood was collected from tail vein to determine the fasting blood glucose （FBG）. Fasting serum insulin （FINS） was detected by orbital blood sampling. And the HOMA-IR index was calculated. The histopathology of heart was invested by HE staining and MASSON staining. The level of COLⅠand COLⅢ in heart were detected by ELISA. Result： ①After 8 weeks of exercise intervention, comparing with C and CE groups, the FBG were significantly higher in DM and DME groups, but FINS were significantly lower. HOMA-IR index were significantly higher in DM and DME groups. comparing with DM group, the level of FBG and HOMA-IR index significantly decreased in DME group, and the FINS significantly increased. Relative heart weight （HW/BW） in DM group and DME group was significantly higher than that in C group, and there was no significant difference between DME group and DM group, CE group and C group. ②fiber fracture ,visible change,necrosis,hyperplasia, disorder arrangement ,edema, degeneration, interstitial congestion were found in myocardium of rats in the DM group severely. Above parameters changed less in DME group and no any in C group and CE group.③Myocardial COLⅠ content in DM group was significantly higher than that in DME group, C group and CE group,and no significant difference between DME group, C group and CE group. myocardial C

关 键 词：2型糖尿病 游泳运动 心肌间质纤维化 COL I COL Ⅲ 

分 类 号：R587.1[医药卫生—内分泌] R493[医药卫生—内科学]