橘核遗传多样性与柠檬苦素类化合物含量的相关性分析  

Correlation analysis between genetic diversity and limonin compounds content of Citri reticulatae Semen

作　　者：胡静[1] 王黎[1] 裴瑾[1] 陈江 吴清华[1] 陈君玉 

机构地区：[1]成都中医药大学中药资源系统研究与开发利用国家重点实验室培育基地,四川成都611137

出　　处：《中草药》2018年第3期678-684,共7页Chinese Traditional and Herbal Drugs

基　　金：国家自然科学基金人才项目(J131003340-13);四川省科技厅项目(2015TD0028)

摘　　要：目的研究橘核种质资源遗传多样性与柠檬苦素类化合物含量的相关性,为筛选橘核的主要基原品种提供参考。方法以四川不同产地、不同品种的35份橘核样品为材料,首先利用NTSYSpc2.10e软件对ISSR标记结果进行多态性分析;再采用UPLC法测定橘核样品中柠檬苦素类化合物(柠檬苦素、诺米林、黄柏酮)的含量;最后通过SPSS 21.0软件对二者数据进行相关性分析。结果筛选出的33条ISSR引物共扩增出240条清晰可辨DNA带,遗传相似系数变化范围为0.72~0.92;其中椪柑与大红袍亲缘关系最近,且柠檬苦素类化合物含量类似,而其余品种橘核与大红袍亲缘关系较远,柠檬苦素类化合物含量差异较大。结论与大红袍亲缘关系越接近的品种其类柠檬苦素含量越高,可考虑将椪柑作为橘核的主要基原品种。Objective To study the correlation between the genetic diversity and limonin compounds content of Citri reticulatae Semen germplasm resources in Sichuan to provide a reference for screening the main source varieties of C. reticulatae Semen. Methods Firstly, NTSYSpc2.10 e software was used to analyze the ISSR markers results of 35 C. reticulatae Semen samples from different habitats and different varieties in Sichuan. Then, UPLC was used to determine the contents of limonin compounds（limonin, nomilin, and obacunone）. Finally, all the results were analyzed by SPSS 21.0 software. Results A total of 240 clear and detectable DNA bands were generated from 33 ISSR primers, and the variation range of genetic similarity coefficient were 0.72—0.92. Citrus reticulate ‘Ponkan＇ and C. reticulate ‘Dahongpao＇ have the closest relationship and their Limonin compounds contents were similar, but the relationship between other varieties and Citrus reticulate ‘Dahongpao＇ was far and limonin compounds contents had great difference. Conclusion The relationship of variety is closer to the C. reticulata ‘Dahongpao＇, the content of limonin compounds is higher, and Citrus reticulate ‘Ponkan＇ could be considered as the main source variety of Citri reticulatae Semen.

关 键 词：橘核 遗传多样性 柠檬苦素类化合物 相关性 基原品种 

分 类 号：R282.12[医药卫生—中药学]