HPLC法同时测定赤芍中9个活性成分的含量  

Simultaneous determination of nine components in Paeoniae Radix Rubra by HPLC

作　　者：王化[1] 何丹娆[1] 朱良玉[1] 于志民[1] 张悦[1] 

机构地区：[1]黑龙江省科学院自然与生态研究所,林下经济资源研发与利用协同创新中心,黑龙江哈尔滨150040

出　　处：《中草药》2018年第3期708-711,共4页Chinese Traditional and Herbal Drugs

基　　金：国家重点研发计划“东北森林区生态保护及生物资源开发利用技术及示范”(2016YFC0500300); 黑龙江省科学院科学研究基金“栽培赤芍配方肥研制与应用”(KY2017ZR01); 黑龙江省科技攻关项目(GZ16C010)

摘　　要：目的建立高效液相色谱(HPLC)同时测定赤芍提取物中9个活性成分(芍药苷、氧化芍药苷、芍药内酯苷、苯甲酰芍药苷、苯甲酸、儿茶素、丹皮酚、没食子酸、1,2,3,4,6-O-没食子酰葡萄糖)的方法。方法采用C18色谱柱(250 mm×4.6mm,5μm);流动相为乙腈和0.1%磷酸水溶液,梯度洗脱,体积流量1.0 m L/min,检测波长230 nm,柱温30℃。结果没食子酸、氧化芍药苷、儿茶素、芍药内酯苷、芍药苷、1,2,3,4,6-O-没食子酰葡萄糖、苯甲酸、苯甲酰芍药苷、丹皮酚进样量分别在0.004~1.200、0.010~1.500、0.044~0.660、0.038~1.140、0.042~2.100、0.050~1.250、0.040~0.600、0.042~1.260和0.004~1.080 mg/m L与峰面积呈良好线性关系,有良好的精密度、稳定性、重复性和回收率。结论该方法同时测定赤芍中9个活性成分的含量高效、准确、重复性好,可用于赤芍药材的质量控制。Objective A high performance liquid chromatographic method（HPLC） was established to simultaneously quantify the paeoniflorin, oxypaeoniflora, albiflorin, benzoyl paeoniflorin, benzoic acid, catechin, paeonol, gallic acid, and 1,2,3,4,6-penta-Ogalloy-D-glucose of Paeoniae Radix Rubra. Methods The mobile phase comprised of acetonitrile and water containing 0.1% phosphoric acid. Column temperature was 30 oC, flow rate was 1.0 m L/min, and chromatography was monitored at 230 nm. Results The correlation coefficients between concentration and chromatographic peak area of gallic acid, oxypaeoniflora, catechin, albiflorin, paeoniflorin, 1,2,3,4,6-penta-O-galloy-D-glucose, benzoic acid, benzoyl paeoniflorin, and paeonol were respectively over 0.999 in the ranges of 0.004—1.200, 0.010—1.500, 0.044—0.660, 0.038—1.140, 0.042—2.100, 0.050—1.250, 0.040—0.600, 0.042—1.260, and 0.004—1.080 mg/m L, with good precision, stability, repeatability, and recovery. Conclusion The method is effective, accurate and reproducible, and can be used for the quality control of Paeoniae Radix Rubra.

关 键 词：赤芍 高效液相色谱法 芍药苷 氧化芍药苷 芍药内酯苷 没食子酰葡萄糖 

分 类 号：R282.6[医药卫生—中药学]