河南省鸡源大肠杆菌的分离鉴定与耐药性分析  

Isolation, Identification and Antimicrobial Resistance of Escherichia coli from Chicken in Henan Province

作　　者：艾伟昌[1] 方忠意 李金磊 董鹏 狄元冉 高延玲 吴志明 王璐[3] 刘振阳[3] 

机构地区：[1]河南省野生动物救护中心,河南郑州450044 [2]河南省兽药饲料监察所,河南郑州450008 [3]河南农业大学牧医工程学院,河南郑州450002

出　　处：《畜牧与饲料科学》2018年第1期4-7,共4页Animal Husbandry and Feed Science

摘　　要：为了掌握河南省鸡源大肠杆菌的耐药性情况,从规模化鸡场抽取样品120批,通过细菌的分离、纯化,采用BD Phoenix^(TM)-100全自动微生物鉴定系统对分离的大肠杆菌进行鉴定,并通过微量肉汤稀释法对分离菌株进行药物敏感性分析。结果显示,共分离菌株100株,分离率为83.3%。分离菌株对四环素、氨苄西林、磺胺异噁唑、恩诺沙星、复方新诺明耐药严重,耐药率均在80%以上,表明河南省鸡源大肠杆菌耐药情况较为严重。The aim of the present study was to characterize the antimicrobial resistance profile of Escherichia coli（E. coli）isolates from chicken in Henan province. A total of 120 samples were recovered from the large-scale chicken farm, and the E.coli stains were isolated and subsequently confirmed by a commercial automated microbiology identification system（BD Phoenix^TM-100）. The susceptibility of the E. coli isolates to the selected antimicrobial agents was determined by broth micro-dilution test. The results showed that a total of 100 E. coli isolates were obtained with the isolating rate of 83.3%; high resistance rate（above 80%） were observed to tetracycline, ampicillin, sulfafurazole, enrofloxacin, and compound sulfamethoxazole. Our investigation demonstrates the prevalence of antibiotic resistant E. coli isolates in chicken farms in Henan Province.

关 键 词：大肠杆菌 分离 鉴定 耐药性分析 

分 类 号：S858.312.6[农业科学—临床兽医学]