不同DNA提取方法对肺癌血浆循环DNA抽提效果比较  

Effect Comparation of Different Extraction Methods for Circulating DNA in Plasma from Patients with Lung Cancer

作　　者：李旺胜 唐一通 张楚微 王书菲 徐洲 杜陈 金钊 

机构地区：[1]湖北文理学院医学院,湖北襄阳441053

出　　处：《教育教学论坛》2018年第11期274-275,共2页Education And Teaching Forum

基　　金：湖北文理学院2017年大学生创新创业训练项

摘　　要：目的:研究不同DNA抽提方法对肺癌血浆循环DNA的提取效果。方法:选取68例肺癌患者为研究对象,比较Gen Mag Bio磁珠法、San Prep柱式PCR产物纯化试剂盒方法、苯酚-氯仿法对肺癌血浆循环DNA的提取效果。结果:几种方法中,磁珠法具有最高的提取效率和最低的CV值。结论:磁珠法对肺癌血浆循环DNA具有最好的抽提效果,适合于对小片段DNA的提取。Objective：To study the effect of different extraction methods＇for circulating DNA in plasma frompatients with Lung cancer, Method：For 68 patients with lung cancer were selected as object. The extraction effect ofGenMagBio magnetic beads,SanPrep column PCtk methods and Phenol--chloroform method was compared forcirculating DNA in plasma from patients with lung cancer. Results：The magnetic beads method had the highestextraction efficiency and the lowest CV value. Conclusion：The magnetic beads method has the best extraction efficiencyfor circulating DNA in plasma from patients with lung cancer, especially suitbale for the extraction of small fragmentDNA.

关 键 词：肺癌 循环DNA 抽提方法 

分 类 号：G642.423[文化科学—高等教育学]