隔药饼灸对高脂血症合并动脉粥样硬化兔肝脏过氧化酶体增殖物激活型受体γ、B类Ⅰ型清道夫受体蛋白及基因表达的影响  

Effect of Herbal-cake-separated Moxibustion on Blood Lipid Levels and Expression of Hepatic PPARγ and SR-B 1 Proteins and Genes in Hyperlipidemia Atherosclerosis Rabbits

作　　者：邹逸凡 马明珠[1] 赵钊[2] 谭静[1] 阳晶晶[1] 石佳[1] 刘密[1] 刘迈兰[1] 常小荣[1] ZOU Yi-fan;MA Ming-zhu;ZHAO Zhao;TAN Jing;YANG Jing-jing;SHI Jia;LIU Mi;LIU Mai-lan;CHANG Xiao-rong(College of Acu-moxibustion and Massage,Hunan University of Chinese Medicine,Department of Acu-moxibustion and Tuina and Rehabilitation,the First Affiliated Hospital of Hunan University of Chinese Medicin)

机构地区：[1]湖南中医药大学针灸推拿学院,长沙410208 [2]湖南中医药大学第一附属医院针灸推拿康复科,长沙410007

出　　处：《针刺研究》2018年第2期86-91,共6页Acupuncture Research

基　　金：国家自然科学基金项目(No.81373716;81704182);国家中医药管理局推广项目;湖南省教育厅项目(No.15C1061)

摘　　要：目的:观察隔药饼灸对高脂血症合并动脉粥样硬化(AS)兔胆固醇逆转运中过氧化酶体增殖物激活型受体γ(PPARγ)、B类Ⅰ型清道夫受体(SR-B 1)蛋白及基因表达的影响,探讨隔药饼灸促胆固醇逆转运抗AS形成的作用机制。方法:新西兰大耳白兔随机分为正常组、模型组、隔药饼灸组、辛伐他汀组,每组10只。采用高胆固醇饲料配合丙基硫氧嘧啶喂养方式制备高脂血症合并AS兔模型。隔药饼灸组予隔药饼灸处理,施灸穴位分为两组,第1组为"巨阙"与双侧"天枢""丰隆",第2组为双侧"心俞""肝俞""脾俞",两组穴位交替使用;辛伐他汀组将辛伐他汀粉(1.96mg·kg^(-1)·d^(-1))拌入第1口饲料;均每天治疗1次,连续4周。测定各组兔血浆总胆固醇(TC)、甘油三酯(TG)、低密度脂蛋白胆固醇(LDL-C)、高密度脂蛋白胆固醇(HDL-C)含量;Western blot法测定肝脏PPARγ、SR-B 1蛋白表达量,RT-PCR法测定肝脏PPARγ、SR-B 1mRNA表达量。结果:与正常组比较,模型组血浆TC、TG、LDL-C均升高(P<0.01),HDL-C降低(P<0.01),PPARγ、SR-B 1蛋白及mRNA表达均降低(P<0.01)。与模型组比较,隔药饼灸组及辛伐他汀组TC、TG、LDL-C均降低(P<0.01),HDL-C均升高(P<0.01),PPARγ、SR-B 1蛋白及mRNA表达均升高(P<0.01,P<0.05)。结论:隔药饼灸调节血脂抗AS的作用机制与增加肝脏中PPARγ的表达、诱导SR-B 1表达,从而促进胆固醇逆转运有关。Objective To observe the effect of herbal-cake-separated moxibustion on blood lipid levels and expression of peroxisome proliferator-activated receptorγ（PPARγ）and scavenger receptor B 1（SR-B 1）proteins and genes in liver of hyperlipidemia atherosclerosis rabbits,so as to explore its mechanism underlying anti-atherosclerosis formation.Methods Forty male New Zealand white rabbits were randomly divided into normal control,model,moxibustion and Simvastatin groups（n=10 rabbits in each group）.The hyperlipidemia atherosclerosis model was established by high cholesterol diet and propylthiouracil for12 weeks.Herbal-cake-separated moxibustion was applied to＂Juque＂（CV 14）,and bilateral＂Tianshu＂（ST 25）,＂Fenglong＂（ST 40）（point group 1）,and bilateral＂Xinshu＂（BL 15）,＂Ganshu＂（BL 18）and ＂Pishu＂（BL 20）（point group 2）.The two groups of points were used alternately.Simvastatin（1.96 mg·kg^（-1）·d^（-1））mixed in the forage was given to rabbits of the Simvastatin group.Both moxibustion and medication treatments were given once daily for continuous 4 weeks.Total cholesterol（TC）,triglyceride（TG）,low density lipoprotein cholesterol（LDL-C）and high density lipoprotein cholesterol（HDL-C）levels in plasma were detected by using an automatic biochemistry analyzer.The expression levels of PPARγand SR-B 1 proteins and genes in the hepatic tissue were determined by Western blot and reverse transcription-polymerase chain reaction,separately.Results After modeling,plasma TC,TG and LDL-C levels in the model group were significantly increased（P0.01）,and the levels of plasma HDL-C and hepatic PPARγand SR-B 1 protein and mRNA expression were obviously down-regulated relevant to the normal group（P0.01）.Compared with the model group,plasma TC,TG and LDL-C levels were significantly decreased（P0.01）,and plasma HDL-C and hepatic PPARγand SR-B 1 protein and mRNA levels were significantly up-regulated in the two treatment groups（P0.01,P0.05）.Con

关 键 词：隔药饼灸 高脂血症 动脉粥样硬化 过氧化酶体增殖物激活型受体γ B类Ⅰ型清道夫受体 

分 类 号：R245.82[医药卫生—针灸推拿学]