E_2RIA系统误差的分析  

Analysis of the Cause of Systematic Error in Estradiol Radioimmunoassay(RIA)

作　　者：彭杨水[1] 李格芳[1] 

机构地区：[1]中山大学附属第一医院胎儿医学中心,510080

出　　处：《放射免疫学杂志》2002年第4期195-196,共2页Journal of Radioimmanology

摘　　要：目的 :通过分析放射免疫测定 (RIA)血清雌二醇 (E2 )的系统误差 ,提高测定的准确度。方法 :用回收实验、溶血和类似物干扰实验及方法比较实验进行系统误差分析。结果 :回收实验 :加入浓度 10 0pg ml回收率 10 1 6 % ,加入浓度 36 0pg ml回收率 10 4 3ng ml。溶血干扰实验 :平均百分干扰值为 - 2 3 36 % ;类似物干扰实验干扰值 :雌二醇 0 19% ,雌酮 13 1% ,孕酮 0 11% ,睾酮 0 4 5 %。1 2 5I-RIA和3H -RIA方法比较实验结果 :Y(1 2 5I-RIA) =16 84 +1 11X(3H -RIA) ,t =11 75 ,p <0 0 5。结论 :1 2 5I-RIA测定血清雌二醇准确度高 ,标本溶血和雌酮影响测定结果 。Objective To improve the accuracy of estradial RIA by analysing the cause of systematic errors. Methods Retrieval assay, hemolysis and analogous substances interference assays and methods-comparing assay were used to analyse the systematic error. Results Retrieval assay: retrieval rates were 101 6% and 104 3% when the adding concentration were 100 0pg/ml and 360 0pg/ml respectively. Haemolysis interference assay: the average interference value was-23 4%. The interference value of analogus substances interference assay were the followings: estriol 0 19%, estrone 13 1%, progesterone 0 11% and testosteone 0 45%. The result of methods-comparing assay ( 125 I-RIA and 3H-RIA) was Y( 125 I-RIA)=16.84+1.11X( 3H-RIA),t=11.75, p <0.05. Conclusion Haemolysis and estrone influence the RIA result to a greater extent. There is systematic error between the two detecting methods and serum estradiol 125 I-RIA is more accurate.

关 键 词：放射免疫分析 雌二醇 系统误差 血清 

分 类 号：R446.61[医药卫生—诊断学]