油茶种仁蛋白双向电泳体系的建立  

Two-dimensional gel electrophoresis system establishment of Camellia oleifera kernel

作　　者：何艺凡 宋倩倩[1] 林文俊[1] 陈世品[1] 陈辉[1] 郑国华[1] 

机构地区：[1]福建农林大学经济林研究所,福建福州350002

出　　处：《森林与环境学报》2018年第1期33-37,共5页Journal of Forest and Environment

基　　金：高等学校博士学科点专项科研基金项目(20123515110010);中央财政林业科技推广示范项目(2017)

摘　　要：以普通油茶优良无性系‘闽43’成熟种仁为材料,从样品制备、IEF聚焦程序参数设定、SDS-PAGE凝胶浓度3个方面优化油茶种仁双向电泳体系。结果表明:采用TCA丙酮+SDS酚抽法提取油茶种仁蛋白能够得到更多较为清晰的蛋白点;采用62 000 V·h的高压进行等电聚焦更适合油茶种仁蛋白的第一向分离;10%SDS凝胶浓度更适合油茶种仁蛋白的双向分离,试验结果为深入开展油茶种仁蛋白质组学的研究奠定基础。Using kernel of Camellia oleifera ‘Min 43＇,a proper two-dimensional electrophoresis system for kernel of Camellia oleifera Abel. was optimized by three factors： sample preparation methods,IEF focusing program parameter settings and SDS-PAGE gel concentration. The results showed that more clear spots could be obtained by the method of TCA/acetone combined with SDS phenol extraction,the isoelectric focusing of 62 000 V·h was more suitable for the separation of protein and the concentration of 10%SDS gel was better for the separation kernel of C. oleifera protein. The result of this research laid the foundation for the following proteomics research in oil tree.

关 键 词：油茶种仁 蛋白 双向电泳 体系 

分 类 号：S664.2[农业科学—果树学]