苦参不同炮制品生物总碱含量测定及HPLC指纹图谱研究  

Research on Contents Alkaloids and HPLC Fingerprints of Different Processed Sophora flavercens Ait.

作　　者：常楚瑞[1] 陆平祝 龙庆德[1] 冉启军 王晓丽[1] 许德勇 

机构地区：[1]贵州医科大学,贵州贵阳550004 [2]重庆市万州区第一人民医院药剂科,重庆404100 [3]贵州省公共资源交易中心,贵州贵阳550004

出　　处：《中国民族民间医药》2018年第4期14-17,共4页Chinese Journal of Ethnomedicine and Ethnopharmacy

基　　金：贵州省中药现代化科技产业研究开发专项项目(黔科合ZY字[2012]3004号);贵州省科技计划项目(黔科合重大专项字[2015]6009-2)

摘　　要：目的:对苦参不同炮制品的生物总碱含量进行测定,并建立苦参生物碱HPLC指纹图谱。方法:采用紫外分光光度法进行苦参生物碱含量测定,采用高效液相色谱法,以Inertsil NH2(5μm,4.6 mm×250 mm)为色谱柱,乙腈-无水乙醇-3%磷酸(84∶10∶6)为流动相,流速为1.0mL/mim,检测波长为210 nm,柱温为30℃。并利用中药色谱指纹图谱相似度评价系统A版软件进行图谱对比。结果:麸制品生物总碱含量最高,为3.955%;炒炭品生物总碱含量最低,为2.604%;各炮制品共有峰的相对保留时间RSD<1.50%,峰面积RSD>26.0%,苦参炮制品指纹图谱相似度在0.231%~0.962%。结论:苦参中主要生物碱成分差异不大,但其量有变化,炮制品的品质不一致,此方法较全面地反映苦参不同炮制品的差异,可用于评价苦参炮制品的质量。Objective Determination of different biologicalcontent of Matrine alkaloids, and establish the HPLC fingerprint of alka- loids in Sophora flavescens Ait. Methods Determination of alkaloids content by UV spectrophotometry, use the method of HPLC, a In- ertsil NH2 （5μm, 4.6 × 250mm） phase column was used with mobile phase consisited of acetonitrle- anhydrod alcohol - 3% acid solution phosphorie （84： 10： 6） at a flow rate ofthe mL/min, the wavelength of detector was hanoi： 3% phosphoric acid as mobile phase, the flow rate is 1.0mL/mim, the detection wavelength is 210nm, the column temperature is nm. the column at 30~C. The ex- perimental data were analyzed by computer aided similarity evaluation software. Results Bran products total alkaloids content was the highest for 3.955%, content of total alkaloids was the lowest which was 2. 604%. There are various processed products peak relative retention time RSD 〈 1.50%, and relative retention peak area RSD 〉 26.0%, Sophora processed products fingerprint similarity in 0. 231% -0. 962%. Conclusion The main alkaloids in Sophora little difference, but its content has changed and inconsistent quality. This method is more comprehensive response to the difference between different Sophora processed products can be used to evaluate the quality of processed products Sophora.

关 键 词：苦参 苦参生物碱 炮制 指纹图谱 质量控制 

分 类 号：R284.1[医药卫生—中药学]