HIV-1中国流行株gag-hIL-2重组痘苗病毒与pIRES-gag-hIL-2核酸疫苗联合免疫的实验研究  

Experimental study of co-immunizations of HIV-1 gag-hIL-2 recombinant vaccinia virus and DNA vaccine pIRES-gag-hIL-2

作　　者：王莉馨[1] 林军 王宏伟[1] 王洪军[1] 金宁一[1] 

机构地区：[1]解放军军需大学病毒学研究所 [2]深圳市科健信息技术有限公司

出　　处：《中国输血杂志》2002年第4期229-231,共3页Chinese Journal of Blood Transfusion

基　　金：国家自然科学基金 (3 9770 66);国家杰出青年基金项目 (3 9770 661)

摘　　要：目的　将HIV 1中国流行株B亚型gag和hIL 2基因在天坛株痘苗病毒中进行共表达 ,与核酸疫苗联合免疫 ,评价免疫效果 ,为新型艾滋病疫苗开发研制奠定基础。方法　将HIV 1中国流行株gag hIL 2基因片段插入到痘苗病毒载体pJ38启动子下游 ,经同源重组和血凝素阴性空斑筛选重组痘苗病毒 ,SDS PAGE、Westernblot检测目的蛋白。以重组病毒和核酸疫苗免疫BALB c小鼠 ,进行淋巴细胞转化实验及血清抗体的细胞免疫和体液免疫指标检测。结果　获得了重组痘苗病毒vJ38gag IL 2 ,具有更好的免疫原性 ,3rVV效果好于 2rVV ,以 2rVV DNA混合免疫效果最好。结论　重组痘苗病毒vJ38gag ILObjective To obtain recombinant vaccinia viruses of gag gene,and hIL 2 gene of HIV 1CN subtype B,evaluate the immune effects after immunization together with nucleic acid vaccine for AIDS vaccine development.Methods gag gene,and hIL 2 gene were inserted downstream of the combined promotor of pJ38 vector.Recombinant vaccinia viruses were selected by using plaque assay.Expression products were examined by SDS PAGE and Western blot.Immune response of BALB/c mice immunized was evaluated by lymphocyte transformation test and OD value of serum IgG antibody.Results Recombinant vaccinia viruses vJ38gag IL 2 were obtained with reactinogenicity.Effect of recombinant vaccinia viruses immunized three times was better than that immunized two times.The best result was obtained in the rVV primed and nucleic acid vaccine boosted group.Conclusion Gag protein and IL 2 protein can be expressed in vJ38gag IL 2

关 键 词：联合免疫 实验研究 HIV-1 中国株gag基因 HIL-2 痘苗病毒 核酸疫苗 

分 类 号：R392－33[医药卫生—免疫学] R512.91[医药卫生—基础医学]