上转换发光技术侧流免疫夹心法检测麻风患者和家庭接触者PGL-Ⅰ抗体水平  

Detection of PGL-Ⅰ antibody in leprosy patients and household contacts by lateral flow immuno-sandwich assay using up-converting phosphor reporter particles

作　　者：张信辉[1] 杨梨丽[1] 杨琴[2] 赵廷芳 杨昌明 戴乾[4] 元康[4] 翁小满[5] 温艳[5] Anouk.van Hooij Paul Corstjens Annemieke Geluk 赖雅芳 付云 李进岚[1] 

机构地区：[1]贵州省疾病预防控制中心,贵阳550004 [2]贵阳市疾病预防控制中心,贵阳550002 [3]黔西南州疾病预防控制中心,兴义562400 [4]安顺市疾病预防控制中心,安顺561000 [5]首都医科大学附属北京友谊医院,北京100050 [6]荷兰莱顿大学医学中心传染病研究所,9990252400 [7]贵州医科大学,贵阳550004

出　　处：《中国麻风皮肤病杂志》2018年第1期4-7,共4页China Journal of Leprosy and Skin Diseases

基　　金：贵州省卫生计生委科学技术基金项目(编号:gzwjkj2014-1-078);国家自然科学基金(编号:81260436);荷兰莱顿大学医学中心传染病研究所麻风病合作项目(编号:MALTALEP)

摘　　要：目的:评估上转换发光技术侧流免疫夹心法(UCP-LFA)检测PGL-Ⅰ抗体对麻风接触者发病风险预测的价值。方法:采用UCP-LFA方法检测贵州59例麻风患者、87例家庭接触者和55例健康对照者的PGL-Ⅰ抗体水平。结果:麻风患者中PGL-Ⅰ抗体阳性率为81.4%、麻风接触者为5.7%、健康对照人群为1.8%,差异有统计学意义(P<0.005)。少菌型和多菌型PGL-Ⅰ抗体阳性率分别为45.5%和89.4%,差异有统计学意义(P<0.005)。BⅠ阳性和BⅠ阴性患者的PGL-Ⅰ抗体阳性率分别为83%和0%,差异有统计学意义(P<0.005)。结论:PGL-ⅠⅠg M抗体水平对麻风发病风险有一定的预测作用。Objective： To evaluate the value of lateral flow immuno-sandwich assay using up-converting phosphor reporter particles（ UCP-LFA） in predicting the risk of leprosy contacts by detecting PGL-Ⅰ antibody. Methods： The PGL-Ⅰ Ig M levels were measured by UCP-LFA in 59 leprosy patients,87 household contacts and 55 healthy controls in Guizhou. Results： The positivity rates of anti-PGL-Ⅰ Ig M in leprosy patients,household contacts and healthy controls were 81. 4%,5. 7% and 1. 8%,with significant differences between them（ P 〈0. 005）. The positivity rates of anti-PGL-Ⅰ Ig M in patients with paucibacillary（ PB） and multibacillary leprosy（ MB） were 45.5% and 89.4%,with a significant difference（ P〈0.005）. The positivity rates of anti-PGL-Ⅰ Ig M in the patients with BI＋ and BI-were 83% and 0%,with a significant difference（ P〈0.005）. Conclusion： PGL-Ⅰ Ig M antibody can predict the risk of leprosy.

关 键 词：上转换发光技术侧流免疫夹心法 麻风 

分 类 号：R755[医药卫生—皮肤病学与性病学]