银杏叶中银杏酸含量的测定  

Determination of Ginkgolic Acids in the Ginkgo Leaf

作　　者：陈君 陈岑 任红 黄海波 乔洪翔 王如伟 

机构地区：[1]云南希康生物科技有限公司,昆明650217 [2]云南康恩贝植物研究院,昆明650217 [3]浙江康恩贝制药股份有限公司浙江省中药制药技术重点实验室,杭州310052

出　　处：《中国现代应用药学》2018年第1期72-75,共4页Chinese Journal of Modern Applied Pharmacy

基　　金："重大新药创制"国家科技重大专项(2011ZX09203-001-16);浙江省科技计划项目(2009E10009)

摘　　要：目的建立高效液相色谱法测定银杏叶中银杏酸含量的方法。方法样品经甲醇超声提取,采用C18柱(4.6 mm×150 mm,5μm),流动相为含0.1%三氟乙酸的乙腈(A)-0.1%三氟乙酸水(B),梯度洗脱(0~30 min,75%→90%A;30~35 min,90%A;35~36 min,90%→75%A;36~45 min,75%A),流速1 m L·min-1,检测波长310 nm。结果样品经甲醇超声提取,快速简便制备银杏酸供试品,白果新酸浓度在0.917~45.85μg·m L-1内呈良好的线性关系(n=6,r2=0.999);平均回收率为97.34%,RSD为0.57%。结论该方法灵敏、准确、简便,适合用于银杏叶中银杏酸含量的测定。OBJECTIVE To develop an HPLC method for the determination of Ginkgolic acids content in the Ginkgo leaf. METHODS The sample was extracted by ultrasonic extraction with methanol as the solvent, the separation was performed on C18 column（4.6 mm×150 mm, 5 μm）, with 0.1% trifluoroacetic acid solution of acetonitrile（A）-0.1% trifluoroacetic acid aqueous solution（B） as mobile phase, and eluted with a gradient program（0-30 min, 75%A→90%A; 30-35 min, 90%A; 35-36 min, 90%→75%A; 36-45 min, 75%A）, flow rate 1 m L·min-1, and UV detection wavelength at 310 nm. RESULTS It was convenient to prepare the sample of Ginkgolic acids by ultrasonic extraction with methanol as the solvent. The linear range for the content of ginkgoneolic acid was 0.917-45.85 μg·m L-1（r2=0.999）, the average recovery was 97.34%, and RSD was 0.57%. CONCLUSION The method is sensitive, accurate and speedy, therefore suitable for the determination of Ginkgolic acids content in the Ginkgo leaf.

关 键 词：银杏酸 银杏叶 含量测定 高效液相色谱法 

分 类 号：R917.101[医药卫生—药物分析学]