植物根围促生细菌菌剂增强玉米干旱耐受性研究  

Enhanced drought tolerance in maize conferred by a consortium of plant growth-promoting rhizobacterium strains

作　　者：王超[1,2] 李刚[2] 谢越盛[1] 高彦林 郭坚华 许泉[1] 

机构地区：[1]南京农业大学,江苏南京210095 [2]环境保护部南京环境科学研究所,江苏南京210042

出　　处：《干旱地区农业研究》2018年第1期126-132,185,共8页Agricultural Research in the Arid Areas

基　　金：江苏省科技厅产学研合作前瞻性项目"防治蔬菜花叶病毒的广谱;高效生物农药创制及关键技术研究"(BY2014128-05);国家重大科技专项项目"东江上游典型集水区水环境风险控制技术集成与综合示范"(2014ZX07206001)

摘　　要：选用植物根围促生细菌(PGPR)菌剂BBS处理玉米幼苗,研究干旱胁迫下玉米幼苗叶片相关生理特性及基因表达差异。结果表明,干旱胁迫15 d后,BBS处理组玉米幼苗的萎蔫程度显著低于对照组;处理组植株叶片MDA含量仅为对照组的74.35%,叶片脯氨酸和可溶性糖含量分别是对照组的3.39倍和1.07倍;处理组植株叶片ZmP5CS1的表达水平及SOD活性均显著高于对照组;BBS处理组植株叶片在干旱胁迫过程中一直保持较低的H_2O_2含量。研究得出,BBS处理能够有效减缓干旱胁迫对玉米幼苗造成的伤害,进而加速正常浇水后的恢复。处理组植株叶片NCED1和ZmDREB2.7的高水平表达也表明干旱胁迫下玉米幼苗与BBS的互作过程可能有依赖ABA信号途径及不依赖ABA信号途径的共同参与。A consortium of plant growth-promoting rhizobacterium( PGPR) strains BBS was demonstrated to enhance tolerance to drought stress in plants. Here,under drought stress,physiological characteristics and transcription of stress-related genes in leaves of maize plants treated with BBS were investigated. After withholding watering for 15 days,BBS-treated maize seedlings had substantially lighter wilt symptoms than control plants. In relation to the control,BBS treatment decreased the leaf monodehydroascorbate( MDA) content by 25.65%,and increased the leaf proline and soluble sugars content by 3.39-fold and 7.28%,respectively. It activated significantly higher transcription of ZmP5CS1 gene,and significantly enhanced the activity of superoxide dismutase( SOD),a major antioxidant enzyme.Additionally,BBS inoculated plants had significantly lower leaf H_2O_2 content during drought stress than the control plants. Consequently,BBS efficiently extenuated the drought-induced injury in maize seedlings and facilitated postdrought recovery. Moreover,the stimulated expression of NCED1 and ZmDREB2.7 indicated the involvement of ABA-dependent and ABA-independent signaling pathways in BBS-maize interaction under water deficit.

关 键 词：玉米 干旱耐受性 植物根围促生细菌 脱落酸 

分 类 号：S513[农业科学—作物学]