工业大麻叶中大麻二酚含量分析研究  

Study on the content of cannabidiol in hemp leaves

作　　者：高宝昌[1] 孙宇峰[1] 张旭[1] 石雨[1] 田媛[1] 崔宝玉[1] 

机构地区：[1]黑龙江省科学院大庆分院,黑龙江大庆163319

出　　处：《黑龙江科学》2018年第1期61-63,共3页Heilongjiang Science

基　　金：黑龙江省院所基本应用技术研究专项;黑龙江省科学院青年创新基金重点项目

摘　　要：建立大麻二酚含量的分析方法,采用Inertsoil ODS-3(150 mm×4.6 mm,5μm)色谱柱,甲醇—水(78∶22,V∶V)为流动相,流速1.0 m L·min-1,检测波长220 nm,柱温30℃的液相色谱方法。考察了提取溶剂种类、提取时间、提取次数、料液比因素对提取效率的影响,最终确定无水甲醇作为提取试剂、超声震荡15 min、料液比为1∶20、提取次数3次的实验方法,该方法简单高效,提取充分,适用于工业大麻叶中大麻二酚成分的定性定量分析。A quantitative analysis method was established for the determination of cannabidiol in hemp leaves via HPLCDAD. The separation of cannabidiol was performed on a Shimadzu ODS-3 column（ 150 mm × 4. 6 mm,5μm） using water-met-hanol（ 78∶ 22,V∶ V） as mobile phase byisocratic elution. The primary quantitative wavelength of UV detector was set at 220 nm,flow rate was set at 1. 0 m L/min,and column temperature was 30℃. The effect of extraction solvent,extraction time, extraction times and solid-liquid ratioon the extraction efficiency was investigated. The optimized conditions are extract with methanol,ultrasound-assisted oscillate for 15 minutes,ratio of sample to solvent 1∶ 20,extraction 3 times. The method was accurate and applicable in the quantitative determination of cannabidiol in cannabis sativa plants.

关 键 词：工业大麻 大麻二酚 含量分析 

分 类 号：TS941.79[轻工技术与工程—服装设计与工程]