CAPS标记开发及果实相关性状QTL分析  

Development of CAPS markers and QTL analysis of fruit-related traits

作　　者：迟莹莹[1] 栾非时[1] 李桂英[1] 

机构地区：[1]东北农业大学,哈尔滨150030

出　　处：《黑龙江科学》2018年第2期1-8,共8页Heilongjiang Science

摘　　要：利用西瓜基因组重测序数据挖掘SNP位点并将其转变为CAPS标记,构建遗传连锁图谱,对西瓜果实相关性状进行QTL分析。以普通西瓜品系(Citrullus lanatus subsp.vulgaris)花园母本和黏籽西瓜品系(Citrullus lanatus subsp.mucosospermus)PI 186490为亲本,构建BC1群体。对亲本材料进行基因组重测序,覆盖西瓜基因组20×以上,发掘SNP位点,运用SNP2CAPS软件选择7种限制性内切酶对包含SNP位点的序列进行酶切位点分析并将其转化为CAPS标记。筛选具有多态性的CAPS标记,对225株BC1群体内各单株进行基因分型,对西瓜果实相关性状进行QTL分析。在两亲本间共获得SNP位点751 532个,设计CAPS引物450对,筛选出多态性引物200对,贡献率为44.44%。所构建遗传连锁图谱包含11个连锁群(分别对应西瓜基因组的11条染色体),覆盖基因组长度1 376.95 cm。获得与果实相关性状QTL位点15个。说明西瓜是可溶性固形物,具有果肉硬度、种子百粒重符合的数量性状遗传特点,种皮底色受四个QTL位点控制。The SNP locus was excised from watermelon genome-heavy sequencing data and transformed into CAPS marker. The genetic linkage map was constructed and the QTLs of watermelon fruit-related traits were analyzed. The BC1 population was constructed from Citrullus lanatus subsp. Vulgaris garden female parent and Citrullus lanatus subsp.Mucosospermus ‘PI 186490'. The parental material was re-sequenced,covering 20 × or more of watermelon genome,exploring SNP loci. Using restriction endonucleases of SNP2 CAPS software,SNP containing SNP sites were analyzed by restriction enzyme digestion and converted into CAPS mark. Polymorphic CAPS markers were screened and 225 BC1 individuals were genotyped for QTL analysis of watermelon fruit-related traits. A total of 751 532 SNPs were obtained from the parents,450 pairs of CAPS primers were designed and 200 pairs of polymorphic primers were screened out,accounting for 44. 44% of the total. The constructed genetic linkage map contains 11 linkage groups(corresponding to11 chromosomes of watermelon genome),covering a genome length of 1 376. 95 cm. Fifteen QTLs for fruit-related traits were obtained. It shows that watermelon is soluble solids with the hardness of the flesh; its number of seeds consistent with the number of 100 genetic characteristics of genetic traits,and seed coat color was controlled by four QTL sites.

关 键 词：西瓜 遗传图谱 SNP CAPS QTL 

分 类 号：S651[农业科学—果树学] Q943.2[农业科学—园艺学]