中国沿海尖刺拟菱形藻的种下分类学研究  

Studies on intraspecies diversity of Pseudo-nitzschia pungens from Chinese coastal waters

作　　者：董焕嫦 黄春秀[1,2] 徐国双 李扬[1,2] 

机构地区：[1]华南师范大学生命科学学院,广州市亚热带生物多样性与环境生物监测重点实验室,广州广东510631 [2]华南师范大学生命科学学院,广东省水产健康安全养殖重点实验室,广州广东510631

出　　处：《热带海洋学报》2018年第1期12-19,共8页Journal of Tropical Oceanography

基　　金：国家自然科学基金(31570205、31370235);广州市科技计划项目(201607010370)

摘　　要：为了澄清中国沿海尖刺拟菱形藻Pseudo-nitzschia pungens Hasle的种下分类学现状,从我国沿海分离并建立了32个尖刺拟菱形藻的单克隆培养株系,结合光学显微镜下的群体特征和透射电镜下的超微形态学特征,以及基于核糖体转录间隔区(ITS)的分子系统学数据,鉴定得到尖刺拟菱形藻的两个变种:尖刺拟菱形藻原变种P.pungens var.pungens和尖刺拟菱形藻阿维罗变种P.pungens var.averiensis。文章对其形态学特征进行了详细描述,并利用高效液相色谱-质谱联用法(LC-MS/MS)对培养株系的多莫酸(DA)进行了检测,但未检测到DA。To declare intraspecies diversity of Pseudo-nitzschia pungens Hasle, a total of 32 monoclonal strains of P. pungensfrom Chinese coastal waters were isolated and established. These strains were identified carefully, based on the morphologyunder both light microscopy and transmission electron microscopy, and on the molecular analysis inferred from the internaltranscribed spacer region sequences. Two varieties were identified： P. pungens var. pungens （Grunow） Hasle and P. pungensvar. averiensis Lundholm, Churro, Carreira, and Calado. Their morphologies were described and compared with allied taxa.Meanwhile, domoic acid （DA） was detected using a liquid chromatography tandem mass spectrometry （LC-MS/MS） injection,but no detectable DA was found. The results may provide detailed information for further studies of toxin production ofChinese Pseudo-nitzschia strains.

关 键 词：尖刺拟菱形藻 种下分类学 形态 核糖体转录间隔区 多莫酸 

分 类 号：P735.531[天文地球—海洋生物学]