草地早熟禾PpGA2ox基因启动子的克隆及功能分析  

Cloning and Functional Analysis of PpGA2ox Gene Promoter from Kentucky Bluegrass

作　　者：檀鹏辉 滕珂 李俊[3] 张兰[1] 尹淑霞[1] 

机构地区：[1]北京林业大学林学院草坪研究所,北京100083 [2]北京草业与环境研究发展中心,北京100097 [3]中国农业科学院草原研究所,内蒙古呼和浩特010010

出　　处：《中国草地学报》2018年第1期1-8,共8页Chinese Journal of Grassland

基　　金：中国博士后基金(2017M620677);北京市博士后基金(2017-ZZ-087)

摘　　要：为了研究草地早熟禾(Poa pratensis L.)GA2-氧化酶基因(PpGA2ox)的启动子,利用染色体步移的方法从草地早熟禾中克隆得到1109bp的PpGA2ox基因启动子序列。Plant CARE在线预测结果表明该启动子序列中存在CAAT-box、TATA-box等启动子基本顺式作用元件以及响应干旱胁迫和茉莉酸甲酯诱导的调控元件。构建PpGA2ox基因启动子与GUS报告基因融合的植物表达载体转化拟南芥,阳性植株GUS组织化学染色结果表明PpGA2ox基因启动子可以驱动GUS基因在拟南芥中表达并且表达具有组织特异性。与对照相比,激素处理及非生物胁迫下GUS基因表达量发生变化,推测PpGA2ox基因的表达可受到激素及非生物胁迫调控。To investigate the promoter of GA2-oxidase gene in Kentucky bluegrass,a 1109 bp promoter sequence of the PpGA2ox gene was cloned using Genome Walking method.Plant CARE analysis revealed that the promoter sequence contained basic cis-acting elements such as CAAT-box,TATA-box and some additional elements involved in drought stress and MeJA-responsiveness.The PpGA2ox promoter and GUS fusion construct was generated and then was successfully transformed into Arabidopsis thaliana.Histochemical analysis of transgenic positive plants revealed that the GUSreporter gene could be driven by PpGA2ox promoter in Arabidopsis thaliana with tissue specificity characters.The expression of GUS gene was varied under hormone treatments and abiotic stresses compared with the control,suggesting that the expression of PpGA2ox could be regulated by hormone and abiotic stresses.This study paved a way to the further study of PpGA2ox.

关 键 词：草地早熟禾 GA2-氧化酶基因 启动子 GUS染色 

分 类 号：S688.4[农业科学—观赏园艺]