多叶苜蓿回交群体遗传结构分析  

Analysis of Population Structure in Backcross Population of Multi-foliate Alfalfa

作　　者：杨艳婷 魏臻武[1] 乔志宏[1] 武自念[2] 

机构地区：[1]扬州大学动物科学与技术学院扬州大学草业科学研究所,江苏扬州225009 [2]中国农业科学院草原研究所,内蒙古呼和浩特010010

出　　处：《中国草地学报》2018年第1期35-41,共7页Chinese Journal of Grassland

基　　金：国家自然科学基金项目"苜蓿多叶性状的遗传特性和基因组学研究"(30972136);江苏省科技支撑计划产学研前瞻性联合研究项目"茶树油对猪生产性能影响及应用效果的研究"(BY2016069-12);中央财政农技推广项目"苕;稻;鸭生态种养技术集成与推广应用"TG(16)008;江苏省普通高校研究生科创计划项目"多叶苜蓿回交群体遗传结构分析"(CXLX13_923;X20160687)

摘　　要：以多叶苜蓿为轮回亲本、以淮阴苜蓿为父本,构建回交群体,通过回交育种获得符合需求的育种材料。对回交一代(BC1)群体的农艺性状与杂交F1群体进行农艺性状遗传多样性分析,并从分子水平进行遗传多样性和群体结构分析。结果表明:BC1群体的农艺性状较F1群体更接近亲本性状,且性状多样性丰富。25对SSR分子标记共检测到148个等位变异,25对引物的平均多态性比率是82.16%。群体结构分析表明,当K=4时ΔK最大,将BC1群体分为4个类群,其中84.27%的单株遗传组分相对比较单一,而15.73%的单株遗传背景比较复杂。多叶苜蓿BC1群体遗传多样性丰富,群体结构的划分与亲缘关系不完全相关。To provide materials for breeding multifoliolate alfalfa varieties that are suitable for Jianghuai area and has higher yields and improved quality,the backcross population was constructed,multi-foliate alfalfa（PL34 HQ）was used as recurrent parent and Huaiyin alfalfa as non-recurrent parent,and breeding materials to meet the requirements for backcross breeding were obtained.The agronomic traits of BC_1 population were compared with F_1 population,the genetic diversity of agronomic traits were analyzed,and genetic diversity and population structure were analyzed from the molecular level.The results showed that agronomic traits of BC_1 population were closer to ones of parents than F_1 population,and traits diversity of BC_1 population were rich.A total of 148 alleles variations were detected by 25 pairs of SSR markers,and average 5.92 per primer,the average ratio of polymorphism was 82.16%.Analysis of population structure showed that K had a maximum value when K=4,the BC_1 could be divided into 4 groups,of which 84.27% of individual plants were with single genetic components,the rest 15.73% were with mixed genetic component.The genetic diversity of BC_1 population were abundant,and the division of population structure were not completely related to genetic relationship.

关 键 词：多叶性状 多叶苜蓿 BC1群体 群体结构 

分 类 号：S541[农业科学—作物学]