银杏叶黄酮组分的分离及抗氧化作用的研究  

STUDY ON THE ANTI OXIDATIVE EFFECT OF DIFFERENT CONSTITUENTS ISOLATED FROM THE TOTAL FLAVONOIDS OF GINKGO BILOBA L.

作　　者：李艳[1] 徐誉泰[1] 张可炜[1] 张举仁[1] 

机构地区：[1]山东大学生命科学学院,济南250100

出　　处：《山东大学学报（自然科学版）》1999年第4期473-478,共6页Journal of Shandong University(Natural Science Edition)

基　　金：山东省科委资助!"银杏叶制药研究"的一部分

摘　　要：用70% 乙醇回流提取和聚酰胺柱层析的方法得到银杏叶总黄酮,继而用硅胶薄板对总黄酮进行制备性分离,得到3 个黄酮含量较高的组分Ⅰ、Ⅱ、Ⅲ.进一步研究发现,组分Ⅰ、Ⅱ、Ⅲ具有较强的抗氧化能力,可以明显地抑制紫外线引起的小鼠肝细胞线粒体过氧化脂质的形成,其中组分Ⅱ的效果最好,当浓度为10 .54 μg/ml 时,抑制率可达60 .3 % .成分结构分析结果表明,组分Ⅱ是以槲皮素为甙元与葡萄糖、鼠李糖或这两者形成的双糖结合而成的黄酮甙类.The total flavonoids was extracted from the leaves of Ginkgo biloba L. by 75% alcohol,purified with polymide column chromatography and separated by TLC.Component Ⅰ、Ⅱ and Ⅲ containing most of the total flavonoids were obtained.Further study showed that flavonoids had a strong anti oxidation effect.They remarkably reduced the level of mitochondria lipid peroxide induced by UV irradiation,and Component Ⅱ was the most effective.When its concentration was 10.54 μg/ml,the inhibitory rate of lipid peroxide reached 60.3 percent.The composition of component Ⅱ was analyzed.The results showed that it mainly consisted of two compounds,both of which were flavone glycosides with quercetin as the aglycon.

关 键 词：银杏叶 黄酮 抗氧化作用 薄层层离 HPLC 过氧化脂质 组分分离 黄酮甙类 

分 类 号：R284.2[医药卫生—中药学] Q946.83[医药卫生—中医学]