免疫球蛋白G对2型糖尿病合并阿尔茨海默病患者外周血单核细胞分泌TNF-α的影响  

Effects of Immunoglobulin G on TNF-α Secretion of Human Peripheral Blood Mononuclear Cells in Type 2 Diabetes Mellitus Complicated with Alzheimer's Disease

作　　者：帅红霞[1] 丁娟 章激[2] 

机构地区：[1]湖北文理学院附属医院内分泌科,襄阳441021 [2]湖北文理学院附属医院药学部,襄阳441021

出　　处：《医药导报》2018年第3期293-296,共4页Herald of Medicine

基　　金：湖北省襄阳市科技局项目(襄科业[2012]40号)

摘　　要：目的探讨免疫球蛋白G对2型糖尿病合并阿尔茨海默病(AD)患者外周血单核细胞(PBMCs)分泌肿瘤坏死因子-α(TNF-α)的影响。方法 2型糖尿病合并AD患者和2型糖尿病患者各20例,密度梯度离心法分离PBMCs,加入植物血凝素(PHA)和2型糖尿病患者血清免疫球蛋白G(Ig G)提取物,酶联免疫吸附(ELISA)法测定培养物的上清液中TNF-α浓度。结果与2型糖尿病组相比,2型糖尿病合并AD组PBMCs培养物的上清液TNF-α浓度明显升高(P<0.01);加入血清免疫球蛋白G提取物后,两组PBMCs培养物上清液TNF-α浓度明显升高(P<0.01)。结论 2型糖尿病患者血清免疫球蛋白G提取物可促进2型糖尿病合并AD患者PBMCs分泌TNF-α,免疫损害可能通过促进炎症反应在2型糖尿病患者发生AD中起作用。Objective To explore the effects of immunoglobulin G on tumor necrosis factor( TNF-α) secretion of human peripheral blood mononuclear cells( PBMCs) in type 2 diabetes mellitus complicated with Alzheimer's disease( AD).Methods A total of 20 cases were chosen in each group from patients with type 2 diabetes mellitus complicated with Alzheimer's disease and patients with type 2 diabetes mellitusm.PBMCs were isolated using density gradient centrifugation method and incubated with phytohemagglutinin( PHA) as well as immunoglobulin G extracted from blood serum of the patients with type 2 diabetes mellitus.TNF-α concentration in supernatant was measured by enzyme linked immunosorbent assay( ELISA). Results TNF-α concentration in supernatant of PBMCs was significantly increased in type 2 diabetes mellitus complicated with AD compared with that in type 2 diabetes mellitus( P<0.01).Ig G further increased TNF-α concentration in type 2 diabetes mellitus and type 2 diabetes mellitus complicated with AD( P<0.01). Conclusion Ig G extracted from the serum of type 2 diabetes mellitus patients can promote TNF-α secretion in PBMCs of type 2 diabetes mellitus complicated with AD. Immunity injury could play a role in the development of type 2 diabetes mellitus complicated with AD by promoting inflammatory reaction.

关 键 词：糖尿病 2型 阿尔茨海默病 单核细胞 肿瘤坏死因子-Α 

分 类 号：R979.5[医药卫生—药品] R58[医药卫生—药学]