精芪双参胶囊对心肌缺血再灌注损伤的影响  

Effect of Jingqi Shuangshen capsule on myocardial ischemia reperfusion injury

作　　者：许艳茹 白冰 于艳辉 徐建 唐晓桐 石菊 周俊佟 

机构地区：[1]吉林修正药业新药开发有限公司,长春130012

出　　处：《长春中医药大学学报》2018年第1期10-13,共4页Journal of Changchun University of Chinese Medicine

基　　金：吉林省科技发展计划项目(20150301009YY);吉林省中药标准化关键工程技术重点实验室

摘　　要：目的观察精芪双参胶囊对心肌缺血再灌注损伤的保护作用,为精芪双参胶囊的开发和应用提供药效学依据。方法采用手术结扎冠状动脉后再灌注方法制备急性心肌缺血动物模型。通过小鼠常压下密闭于250 mL的广口瓶内,记录小鼠存活时间。用体内血栓电刺激法观察大鼠体内血栓形成情况。结果精芪双参胶囊可明显抑制心脏指数及心电图ST段的位移变化(P<0.05),明显抑制心肌梗死面积扩大,保护心肌细胞、减少心肌纤维间质中红细胞沉积及炎细胞浸润,可明显抑制大鼠血清中CK、AST、LDH、MDA含量的释放,并提高SOD活力变化(P<0.05),促进血浆中PGI2释放并抑制TXA2的释放,同时升高PGI2/TXA2比值(P<0.05);明显延长小鼠常压耐缺氧时间(P<0.05);明显抑制成栓100%的时间和血栓长度增加(P<0.05)。结论精芪双参胶囊可以明显改善大鼠缺血缺氧,对心肌缺血有较好的保护作用,并可以抑制血小板聚集,抑制栓塞。Objective To observe the protective effect of Jingqi Shuangshen capsule on the ischemia myocardial,and to provide the pharmacodynamic basis for the development and application of Jingqi Shuangshen capsule.Methods An animal model of acute myocardial ischemia was prepared by ligating the coronary artery and reperfusion.The survival time of mice was recorded under the condition that the mice were sealed in a jar of 250 mL at atmospheric pressure.Thrombus formation in rats was observed by electric stimulation of thrombus in vivo.Results Jingqi Shuangshen capsule can obviously inhibit the cardiac index and electrocardiogram ST segment changes in displacement（P〈0.05）;Myocardial infarct size was significantly inhibited,myocardial cells were protected,blood cell deposition and inflammatory cell infiltration were reduced in the interstitium of myocardial fibers;It significantly inhibited the release of CK,AST,LDH and MDA in serum,and improved the activity of SOD（P〈0.05）;It promotes the release of PGI2 in plasma and inhibits the release of TXA2,while increasing the ratio of PGI2/TXA2（P〈0.05）.It significantly prolong the time of hypoxia tolerance（P〈0.05）;And significantly inhibited the time of thrombosis 100% and the length of thrombus（P〈0.05）.Conclusion Jingqi Shuangshen capsule can significantly improve the ischemia and hypoxia of rats,protect myocardial ischemia,inhibit platelet aggregation and embolism.

关 键 词：精芪双参胶囊 心肌缺血再灌注损伤 血栓素(TXA2) 前列环素(PGI2) 血栓 

分 类 号：R285.5[医药卫生—中药学]