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作 者:周玉杰[1,2] 李建华 张广宇[1] 王宁[1] 谭文丽[1] 王永鹏 王春燕[1] 曹启民[1]
机构地区:[1]海南省农垦科学院,海南海口570206 [2]海南省农垦农业科学研究所,海南海口570311
出 处:《广东农业科学》2017年第11期68-74,共7页Guangdong Agricultural Sciences
基 金:海南省自然科学基金(317204);国家现代农业产业技术体系建设专项资金(CARS-34-GW10;CARS-34-ZP3);海南省重点研发计划项目(ZDYF2016105);海南耕地改良关键技术研究与示范专项(HNGDhs 2015);海南农业遥感技术推广应用与示范研究项目(ZDKJ2016021-02-05)
摘 要:为了解橡胶林土壤微生物群落功能多样性特征,运用Biolog-ECO技术对海南省大丰农场6个不同林龄橡胶林割胶前后土壤微生物碳代谢功能多样性进行研究。结果表明:随着培养时间延长,土壤微生物碳代谢活性增强,培养144 h后趋于平稳。橡胶林土壤微生物利用羧酸类、氨基酸类、胺类和糖及衍生物类碳源能力较强,而对酚酸类和多聚物类碳源利用能力较差;除未开割的林龄5年橡胶林外,林龄10、13、18、25、30年橡胶林割胶后(11月)土壤微生物AWCD值、Shannon-Wiener指数、Mc Intosh指数和利用碳源能力均低于割胶前(5月),说明割胶引起了橡胶林土壤微生物碳代谢功能多样性下降。不同林龄橡胶林土壤微生物AWCD值、Shannon-Wiener指数、Mc Intosh指数和利用碳源能力的差异在割胶前后均不同。The functional diversity soil microbial carbon metabolism and its changes at different aged rubber tree plantations in Dafeng farm of Hainan province were studied by Biolog-eco technique. The results indicated that: the carbon metabolic activity of soil microbial increased with the extend of culture time,and its tend to was stable after 144 hours. The utilization carbon capacity of soil microbial communities in rubber tree plantations was strong for the sources of carboxylic acids,amino acids,amines and carbohydrates,but the capacity of using phenolic acids and polymers was poor. The average well color development(AWCD),Shannon-wiener index,Mc Intosh index and capacity of utilization carbon source in tapping after(November) were lower than those in indicated tapping before(May). This that tapping caused the functional diversity of soil microbial carbon metabolism of rubber tree plantations decreased.There were differences of average well color development(AWCD),Shannon-wiener index,Mc Intosh index and capacity of utilization carbon source at different ages of rubber tree plantations in tapping before and after.
关 键 词:橡胶林 土壤微生物 Biolog-ECO技术 功能多样性
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