维生素C片剂和针剂的间接分光度测定  

Indirect Spectrophotometric Determination of Vitamin C in Tablets and Injections

作　　者：汤泉[1] 韦正友[1] 张晖[1] 王杰[1] 

机构地区：[1]蚌埠医学院,安徽蚌埠233030

出　　处：《广州化工》2018年第3期92-94,共3页GuangZhou Chemical Industry

基　　金：安徽高校自然科学研究重点项目(KJ2016A485)

摘　　要：采用邻二氮菲作显色剂间接分光光度测定片剂和针剂中维生素C的含量。在酸性介质中铁(Ⅲ)被维生素C定量还生成铁(Ⅱ),二价铁可与邻二氮菲作用生成橙红色配位化合物,此配位化合物最大吸收波长510 nm,且浓度与吸光度成正比。本法以标准铁溶液代替标准维生素C溶液,无需对维生素C进行纯制和标定,此方法所用仪器普通,具有简单、快速、灵敏度高的特点。An indirect spectrophotometric method for the determination of vitamin C in table and injection was reported,using 1,10-phenanthrooline as chromogenic reagent. In acid medium,iron( Ⅲ) was quantitatively reduced to yield iron( Ⅱ) by vitamin C,and the latter reacted with 1,10-phenanthroline to yield an orange-red complex compound.The orange-red species had an maximum absorption wavelength at 510 nm,and the concentration of the orange-red species was proportional to the absorbance. In this method,the standard vitamin C solution was replaced by the standard iron( Ⅲ) solution,so purification and standardization of vitamin C were not required. The instrument was common. The method has the advantages of simple,rapid and high sensitivity.

关 键 词：间接分光光度分析 维生素C 邻二氮菲 标准铁(芋)溶液 

分 类 号：O657.34[理学—分析化学]