高迁移率族蛋白1在刺激人肾系膜细胞增殖并分泌纤维化相关因子中的作用  

Effect rHGMB1 on proliferation of human renal mesangial cells and secretion of fibrosis-related cytokines.

作　　者：周健光 陈瑜[1] 

机构地区：[1]浙江中医药大学附属第一医院全科医学科,浙江杭州310019

出　　处：《医药前沿》2017年第36期16-19,共4页Journal of Frontiers of Medicine

基　　金：浙江省自然科学基金资助项目（LQ13H100004）

摘　　要：目的：探讨高迁移率族蛋白1（high mobility group prptein，HMGBI）在促使人肾系膜细胞（human tenal mesangial cell，HRMCs）增殖及分泌纤维化相关因子中的作用。方法：用不同浓度梯度的重组HMGB1刺激体外培养的人肾系膜细胞，以CCK-8测定人肾系膜细胞增殖情况，以ELISA方法检测培养上清液中纤维化相关因子TGF-β1的分泌量，以real-timePCR法测定细胞周期蛋白cyclinD、PNCA蛋白的表达。结果：一定浓度的HMGB1可刺激人肾小球系膜细胞的增殖，细胞内细胞周期蛋白cyclinD、PNCA基因表达增加，细胞培养上清液中TGF-β1浓度增高。结论：HMGBI通过刺激人肾小球系膜细胞增殖并分泌TGF-β1参与了肾脏纤维化过程。Objective To investigate the role of HMGB1 in inducing human renal mesangial ceils {HMCs） to proliferate and secret fibrosis-related cytokines. Methods Recombinant protein HMGB1 （rHMGB1） was incubated with HMCs and then proliferation assay was carried out with CCK-8; cyclinD and PCNA genes were determined with real time PCR; supernate of cultured HRMCs with rHMGB1 was examined for detection of TGF-β1 with ELISA method. Results HMGB1 promoted proliferation of HRMCs; HMGB1 increased expression of cyclinD and PCNA genes involving cell proliferation; rHMGB1 increased secretion of TGF-β1 in supernate of cultured HMCs. Conclusion rHMGB1 was involved in fibrosis of kidney by inducing HRMCs to proliferate and secret fibrosis-related cytokines.

关 键 词：高迁移率族蛋白1 人系膜细胞 纤维化 TGF-Β1 增殖 细胞因子 

分 类 号：R68[医药卫生—骨科学]