解毒护肝颗粒对免疫性肝损伤大鼠炎症因子和小肠紧密连接蛋白的影响  

Effects of Jiedu hugan granule on the inflammatory factor and tight junction protein of small intestine in rats with immunological liver injury

作　　者：徐赛华 胡静娜 梅璟妍 马卫成 

机构地区：[1]宁波市鄞州第二医院中药房 [2]宁波市鄞州第二医院药剂科

出　　处：《中国临床药理学与治疗学》2017年第12期1352-1357,共6页Chinese Journal of Clinical Pharmacology and Therapeutics

基　　金：宁波市自然科学基金项目(2012A610199)

摘　　要：目的:研究解毒护肝颗粒对免疫性肝损伤大鼠炎症因子和小肠紧密连接蛋白的影响。方法:随机将50只SD大鼠分为对照组,模型组,解毒护肝颗粒低、中、高3个剂量(2.7、5.4和10.8g/kg)实验组。除对照组外,其余各组采用腹腔注射猪血清0.5 mL/只,建立免疫性肝损伤大鼠模型。灌胃给药1次/d,连续给药14 d。通过苏木精-伊红(HE)染色法观察肝组织及小肠组织病理变化,检测血清丙氨酸转氨酶(ALT)、天门冬氨酸转氨酶(AST)和乳酸脱氢酶(LDH)。免疫组化法检测肝组织肿瘤坏死因子(TNF-α)和小肠组织occludin-5表达。结果:模型组肝组织出现脂肪细胞堆积,肝细胞增大,肝索排列紊乱,伴炎性细胞浸润;实验组肝细胞形态尚规则,肝索排列整齐,炎性细胞浸润明显减轻。模型组小肠黏膜绒毛萎缩,上皮细胞脱落,水肿明显,大量炎性细胞浸润;实验组黏膜紧密,水肿减轻,浸润的炎性细胞不明显。试验组ALT、AST、LDH和TNF-α显著低于模型组,且随解毒护肝颗粒剂量的增加,试验组ALT、AST、LDH和TNF-α显著降低;而试验组occludin-5显著高于模型组,且随解毒护肝颗粒剂量的增加,试验组occludin-5显著升高,差异有统计学意义(P<0.05)。结论:解毒护肝颗粒可改善肝及小肠的组织结构,保护损伤的肝脏,减轻炎症反应,维持肠黏膜屏障功能。AIM： To investigate the influence of Jiedu hugan granule on the inflammatory factor and tight junction protein of small intestine in rats with immunological liver injury.METHODS： Fifty SD rats were randomly divided into control group,model group,low,middle and high experimental group of Jiedu hugan granule（ 2.7,5.4,and 10.8 g/kg）.All the groups excepted the control group received intraperitoneal injections of 0.5 mL pig serum for each to establish immunological liver injury model.The experimental group was irrigated 1 time/d,continuous dosing for 14 d.The liver tissue and small intestinal tissue pathological changes were observed by HE staining,the serum alanine aminotransferase（ ALT）,aspartate aminotransferase（ AST） and lactate dehydrogenase（ LDH） were detected,and the expression of liver tissue TNF-α and small intestine tissue occludin-5 were detected by immunohistochemical method.RESULTS： The model group liver tissue showed accumulation of fat cells,liver cells enlargement,disorganized liver and associated with inflammatory cells infiltration; the experimental group liver cell morphological ruled,hepatic cords in alignment and inflammatory cells infiltration significantly decrease.The model group small intestinal mucosa villi showed atrophy,fall off of epithelial cell,edema,large number of inflammatory cells infiltration; the experimental group showed closelyknitted small intestinal mucosa villi,relieve edema,and no obvious infiltration of inflammatory cells.The levels of ALT,AST,LDH and TNF-α in experimental group were statistically lower than the model group,and the levels were statistically reduce with the dose of jiedu hugan granule increased.The levels of occludin-5 in experimental group were statistically highter than those of the model group,and the levels of occludin-5 were statistically increase with the dose of Jiedu hugan granule increased, the differences were statistically significant（ P〈0.05）.CONCLUSION： Jiedu hugan granule can improve the organizational structure

关 键 词：保肝解毒颗粒 免疫性肝损伤 炎症因子 紧密连接蛋白 肠黏膜屏障 

分 类 号：R965.2[医药卫生—药理学] R285.5[医药卫生—药学]