雷公藤多苷增强耐顺铂人上皮性卵巢癌SKOV3/DDP细胞株顺铂敏感性的体外研究  

Study on the cisplatin-sensitization effect of tripterygium glycosides on cisplatin-resistant human epithelial ovarian cancer cells (SKOV3/DDP) in vitro

作　　者：刘文成[1] 谭布珍[1] 占欣璐 胡辉[1] 黄根华[1] 曹青[2] 

机构地区：[1]南昌大学第二附属医院,妇产科,江西南昌330006 [2]南昌大学第二附属医院,分子实验中心,江西南昌330006

出　　处：《中国临床药理学与治疗学》2017年第12期1364-1370,共7页Chinese Journal of Clinical Pharmacology and Therapeutics

基　　金：江西省自然科学基金(20161BBG70218);江西省卫生计生委中医药管理局科研基金(2016A078)

摘　　要：目的:探讨雷公藤多苷(GTW)对耐顺铂人上皮性卵巢癌细胞株(SKOV3/DDP)的顺铂(DDP)增敏作用及机制。方法:将对数生长期的SKOV3/DDP细胞随机分为8组:空白对照组、10μg/mL DDP组、50μg/mL GTW组、800μg/mL GTW组、3 200μg/mL GTW组、10μg/mL DDP+50μg/mL GTW组、10μg/mL DDP+800μg/mL GTW组、10μg/mL DDP+3 200μg/mL GTW组,利用CCK-8法、流式细胞术、Western blot法检测各组细胞生长、细胞凋亡以及细胞谷胱甘肽S转移酶-π(GST-π)基因蛋白、P-糖蛋白(MDR1)、信号转导和转录激活因子3(STAT3)、p-STAT3的表达。结果:DDP与GTW联用在抑制SKOV3/DDP细胞生长作用上呈现相加效应;800、3 200μg/mL GTW分别与10μg/mL DDP联用后,可显著促进SKOV3/DDP细胞凋亡,下调p-STAT3的表达(P<0.05)。结论:GTW可显著增强SKOV3/DDP细胞对DDP的敏感性,其作用机制可能是下调STAT3信号通路中的p-STAT3表达。AIM： To study the cisplatin-sensitization effect and mechanism of tripterygium glycosides on cisplatin-resistant human epithelial ovarian cancer cells（ SKOV3/DDP）.METHODS： The SKOV3/DDP cells in exponential phase of growth were randomly divided into eight groups： blank control group,10 μg/mL DDP group,50 μg/mL GTW group,800 μg/mL GTW group,3 200 μg/mL GTW group,10 μg/mL DDP ＋ 50 μg/mL GTW group,10μg/mL DDP ＋ 800 μg/mL GTW group and 10 μg/mL DDP ＋ 3 200 μg/mL GTW group.Cell counting kit 8 and flow cytometry and western blot were used to detect the growth inhibiting rate and apoptosis rate and relative expression of GST-π,MDR1,STAT3,p-STAT3 of SKOV3/DDP cells of every group.RESULTS： DDP and GTW produce an additive effect when used concurrently in inhibiting growth of SKOV3/DDP cells.800 μg/mL GTW or 3 200 μg/mL GTW combined with 10 μg/mL DDP can significantly induce apoptosis of SKOV3/DDP cells and downregulate the expression of p-STAT3（ P〈0.05）.CONCLUSION： GTW can significantly enhance sensitivity of SKOV3/DDP cells to DDP.The underlying mechanism may be related with down-regulating the expression of p-STAT3 in STAT3 pathway.

关 键 词：雷公藤多苷 SKOV3/DDP 顺铂 增敏作用 STAT3 

分 类 号：R965.2[医药卫生—药理学] R737.31[医药卫生—药学]