西瓜鸟氨酸氨甲酰转移酶基因克隆及原核表达  

Cloning and Prokaryotic Expression of Ornithine Carbamoyl Transferase from Watermelon

作　　者：贺滉[1] 牛美丽 申龙斌[1] 詹园凤[1] 党选民[1] 

机构地区：[1]中国热带农业科学院热带作物品种资源研究所,儋州571737 [2]舞阳县第一高级中学,舞阳462400

出　　处：《分子植物育种》2017年第12期4847-4853,共7页Molecular Plant Breeding

基　　金：中央级公益性科研院所基本科研业务费专项项目(1630032015016);中国热带农业科学院院级创新团队项目(17CXTD-02)共同资助

摘　　要：从西瓜叶片中克隆瓜氨酸合成途径的关键酶鸟氨酸氨甲酰转移酶(OCT)基因。OCT基因由1 137个碱基组成,编码378个氨基酸,蛋白分子量约为41.8 k D,等电点为8.61。氨基酸序列比对分析表明,西瓜OCT与香瓜和黄瓜等物种的OCT基因同源性较高,分别为93%和92%;进化树分析发现,西瓜与黄瓜亲缘关系最近,而与狭刀豆亲缘关系最远。构建p ET-30a-OCT原核表达载体,转化大肠杆菌BL21(DE3),经IPTG诱导和SDS-PAGE检测,表达的融合蛋白与预期蛋白大小相符合。本研究为进一步研究其酶学特性和瓜氨酸合成代谢中的作用提供技术支持。Cloned the Ornithine Carbamoyl transferase gene（OCT）, a key gene in the citrulline synthesis pathway,from watermelon leaves. The gene was composed of 1 137 bases, encoded 378 amino acids with molecular weight41.8 k D and p I 8.61. Amino acid sequence analysis showed that watermelon OCT had 93% and 92% high identity with muskmelon and cucumber. The phylogenetic tree analysis showed that the relationship between watermelon and cucumber was the closest, The farthest Canavalialineata DC. The fusion protein expressed by IPTG induction and SDS-PAGE detection was consistent with the expected size of the protein. This study laid the foundation for further research of its enzymatic characteristics and the role of citrulline biosynthesis.

关 键 词：西瓜 OCT 克隆 原核表达 

分 类 号：S651[农业科学—果树学]