不同长度AlsCBF基因启动子片段分离及瞬时表达分析  

Isolation and Post-transient Expression Analysis of Different Length AlsCBF Promoters

作　　者：陈婷婷[1] 余曦瑶 任燕萍[1] 罗淑萍[1] 郭淑朋 李疆[1] 

机构地区：[1]新疆农业大学,乌鲁木齐830052

出　　处：《分子植物育种》2017年第12期4940-4946,共7页Molecular Plant Breeding

基　　金：国家自然科学基金(31660562);新疆自治区园艺学重点学科基金(2016-10758-3)共同资助

摘　　要：CBF基因在冷驯化过程中发挥重要的作用。本研究依据已克隆的新疆野扁桃(Amygdalus ledebouriana Schleche)CBF基因启动子顺式作用元件的分布,构建ACpro-P1(1 110 bp)、ACpro-P2(923 bp)、ACpro-P3(654 bp)和ACpro-P4(350 bp)4个5'缺失植物表达载体;利用烟草叶片注射法瞬时表达分析后进行GUS组织染色分析和定量分析。结果显示这4个片段均具有诱导型启动子活性,启动子Als CBF在-1 391^-1 110 bp之间并无与低温、高盐、干旱三类逆境密切相关的启动子元件;-1 110^-654 bp之间存在大量与低温响应相关的启动子元件;-1 110^-923 bp之间存在能够响应高盐胁迫的元件,而-923^-350 bp之间可能存在一些负调控元件,降低了启动子对高盐的响应;-923^-654 bp之间存在大量与干旱胁迫有关的响应元件。研究结果为进一步分析Als CBF的功能奠定基础。CBF gene plays a fundamental role in cold acclimation. The study, based on the distribution of ciselement in the promoter of Xingjiang almond（Amygdalus ledebouriana Schleche） CBF gene, builded ACpro-P1（1 110 bp）, ACpro-P2（923 bp）, ACpro-P3（654 bp） and ACpro-P4（350 bp） four 5＇deletion plant expression vectors and used post-transient GUS gene expression analysis in tobacco leaves after histochemical staining analysis and quantitative analysis. The result showed that all four fragments had an inducible promoter activity, and the promoter AlsCBFpro did not have promoter element related with the low temperature, high salt, and drought between-1 391^-1 110 bp. There were a large number of promoter element associated with low temperature between-1 110^-654 bp. There were promoter element responding to high salt stress between-1 110^-923 bp while there might be some negative regulatory elements between-923^-350 bp, reducing the promoters of high in response to salt. There were promoter element associated with drought stress between-923^-654 bp. These results provided reference for further analysis on the functional characterization of AlsCBF.

关 键 词：野扁桃 CBF启动子 GUS活性 

分 类 号：Q943.2[生物学—植物学]