表油菜素内酯诱导下粗枝云杉胚性组织的生理响应  

Physiological Characteristics Responses of Picea asperata Mast. Embryogenic Tissue under Epibrassinolide Induction

作　　者：苗晓娟 夏燕[1,2] 景丹龙 麻文俊[1] 杨桂娟 王军辉 

机构地区：[1]中国林业科学研究院林业研究所,国家林业局林木培育重点实验室,国家林木种质资源平台,北京100091 [2]西南大学园艺园林学院,重庆400716

出　　处：《分子植物育种》2017年第12期5038-5043,共6页Molecular Plant Breeding

基　　金：“十二五”国家科技支撑项目(2012BAD01B01)资助

摘　　要：为了探索粗枝云杉胚性组织对表油菜素内酯的生理生化响应,本研究以粗枝云杉3个基因型未成熟胚为供试材料,通过在诱导培养基中添加不同浓度EBR(0,0.5μmol/L,1.0μmol/L),经诱导后获得胚性组织,然后进行增殖培养,在同一增殖周期的第0 d和第10 d时,比较胚性组织中超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)的活性以及丙二醛(MDA)和可溶性蛋白含量的变化,结果表明,实验组与对照组相比,在第0天时,胚性组织中POD活性、CAT活性、MDA含量以及可溶性蛋白含量均升高了,而SOD活性降低了;在第10天时,胚性组织中SOD活性、CAT活性、MDA含量以及可溶性蛋白含量均升高了,而POD活性降低了。研究结果为探讨粗枝云杉胚性组织的生理生化变化建立基础资料,也为提高粗枝云杉胚性组织诱导率提供参考依据。The study investigated the interaction relationship between different genotypes embryonic tissues of Picea asperata Mast. under epibrassinolide（EBR） induction. Embryonic tissues were induced from immature zygotic embryos of three genotypes of P. asperata by adding different concentrations EBR（0, 0.5 μmol/L, 1.0 μmol/L） in the induction medium. In the same proliferation cycle at the 0 and 10 d, the different activities of superoxide dismutase（SOD）, peroxidase（POD）, catalase（CAT）, as well as the contents of malondialdehyde（MDA） and soluble protein were examined. The results showed that compared with the control group, on the 0 th d the POD, CAT activity and MDA, soluble protein content of experimental group were higher, but the activity of SOD was lower; on the 10 th d the SOD, CAT activity and MDA, soluble protein content of experimental group were higher, but the activitie of POD was lower. The results would establish a basic data for the biochemical and physiological changes study of embryonic tissues, and provide evidence for the improvement of embryonic tissue induction rate of P. asperata.

关 键 词：粗枝云杉 表油菜素内酯 胚性组织 生理特性 

分 类 号：S791.18[农业科学—林木遗传育种]