稻瘟病原菌对水稻突变体ospfa-dsp2生长及抗氧化酶活性的影响  

Effect of Magnaporthe grisea on Growth and Antioxidant Enzyme Activity of Rice Mutant ospfa-dsp2

作　　者：何含杰[1,2] 唐丽[1] 米强 邓华凤[1,3] 

机构地区：[1]杂交水稻国家重点实验室,湖南杂交水稻研究中心,长沙410125 [2]中南林业科技大学林业生物技术湖南省重点实验室,湖南省生物发育工程及新产品研发协同创新中心,长沙410018 [3]南方粮油作物协同创新中心,长沙410125

出　　处：《分子植物育种》2017年第12期5079-5084,共6页Molecular Plant Breeding

基　　金：湖南省自然科学基金(2015JJ3178);国家自然科学基金(31571259);杂交水稻国家重点实验室开放性项目(2014KF03)共同资助

摘　　要：蛋白酪氨酸磷酸酶在植物的生长发育和胁迫应答过程中发挥着重要的功能,已有的报道表明Os PFA-DSP2是一个蛋白酪氨酸磷酸酶,在稻温病原菌的应答过程中发挥负调控作用。为了研究稻温病菌(Z1菌株)对突变体ospfa-dsp2生长和抗氧化酶活性的影响,试验以水稻为材料,利用分光光度计测定抗氧化酶活性、抗坏血酸和谷胱甘肽的含量,利用荧光定量PCR仪检测抗病基因的表达水平。试验结果显示,稻瘟病原菌对野生型日本晴水稻和ospfa-dsp2突变体的生长无显著性影响,但可以提高体内抗病基因的表达水平;稻瘟病原菌可以提高ospfa-dsp2突变体叶片中POD和SOD活性水平,降低抗坏血酸的含量,说明Os PFA-DSP2缺失后可以提高植株对稻温病菌的抗性。Protein tyrosine phosphatases play key role in the processes of growth, development and stresses response in plant, the previous reports showed that Os PFA-DSP2 is a protein tyrosine phosphatase, and play negative regulation in Magnaporthe grisea response. To study the effect of Magnaporthe grisea（Z1 strain） on growth and antioxidant enzyme activities, using the rice as material, the antioxidant enzyme activities, the contents of ascorbic acid and glutathione were determined by spectrophotometer, the genes of pathogenesis resistance（PR） were detected by real time quantitative polymerase chain reaction（q RT-PCR）. The results showed that the effect of Magnaporthe grisea Z1 on the growth of wild type rice and mutant ospfa-dsp2 was not significant. Compared with control, the activity levels of POD and SOD in leaves were increased obviously in mutant, and the content of ascorbic acid in leaves was decreased. These results indicated that deletion of Os PFA-DSP2 could enhance the resistance to Magnaporthe grisea response.

关 键 词：水稻 稻瘟病原菌 抗氧化酶 抗坏血酸 谷胱甘肽 

分 类 号：S435.111.41[农业科学—农业昆虫与害虫防治]