深度单采浓缩红细胞对机体造血功能影响的动物实验  

An experimental study of advanced autologous concertrated red blood cells apheresis and its impact on hematopoiesis

作　　者：王丽华[1] 陈冠伊[1] 吴靖辉[1] 刘圣娥 陈蕾 杨金华[1] 谷立男 卢祝杰 欧阳锡林[1] 

机构地区：[1]解放军总医院第一附属医院输血科,北京100048 [2]承德市第三医院 [3]吉化集团公司总医院第二附属医院

出　　处：《中国输血杂志》2017年第12期1324-1326,F0001,共4页Chinese Journal of Blood Transfusion

基　　金：北京市科技计划课题(Z151100003915108)

摘　　要：目的采用动物实验了解深度采集浓缩红细胞对于机体造血功能的作用。方法选取16只健康成年雄性山羊,随机分组法等分为深度采血(实验A)组:采集浓缩红细胞(150-250)mL/只;采集全血(对照B)组:常规采集全血150 mL/只。2组动物分别于采血前1 d与采血后1、3、7、14、21 d各抽取静脉血10 mL/只,检查血常规、铁代谢指标及EPO等,观察、比较2组动物各项指标的变化;同时于采血前1 d与采血后7、14、21 d做髂后上嵴骨髓穿刺术取骨髓涂片检查,观察、比较2组动物骨髓造血能力的变化。结果 A、B 2组动物采血后1、3、7、14、21 d的Hb(g/L)分别为:97.9±9.2 vs 107.1±8.5、95.8±6.5 vs 106.1±10.6(P<0.05)、97.9±8.4 vs 105.4±12.0、107.5±8.9 vs 109.8±12.0、112.5±8.1 vs 114.0±17.9,其中2组动物采血后1、3 d均明显低于采血前:97.9±9.2 vs 95.8±6.5 vs 105.5±11.8、107.1±8.5 vs 106.1±10.6 vs 112.4±11.7(P<0.05);2组动物采血前后铁代谢指标及EPO的变化无明显变化(P>0.05),骨髓细胞均为增生活跃。结论深度采血组动物造血启动速度、造血速度大大高于采集全血组动物。Objective To evaluate the impact of advanced autologous concertrated red blood cells（ CRBC） apheresis on hematopoiesis. Methods 16 healthy adult male goats were randomly divided into group A and group B. 150-250 mL（ CRBC） were collected from each individual subject in group A,while 150 mL whole blood per goat was collected in group B. The routine blood tests,iron metabolism index and EPO tests were conducted on both groups preoperatively and at d1,d3,d7,d14,d21 post-operation. Bone marrow examination was performed as well preoperatively and at d1,d7,d14,d21 post-operation. Results The levels of hemoglobin（ g/L） in two groups at d1,d3,d7,d14 and d21 post operation were（ A vs B） 97. 9±9. 2 vs 107. 1±8. 5、95. 8±6. 5 vs 106. 1±10. 6（ P = 0. 034） 、97. 9± 8. 4 vs 105. 4± 12. 0、107. 5± 8. 9 vs 109. 8±12. 0、112. 5±8. 1 vs 114. 0±17. 9. The two groups both present noticeable lower hemoglobin values at d1 and d3 compared to preoperative values： 97. 9±9. 2 vs 95. 8±6. 5 vs 105. 5±11. 8、107. 1± 8. 5 vs 106. 1 ± 10. 6 vs 112. 4 ± 11. 7（ P 〈0. 05）. The levels of iron metabolism index and EPO in two groups possess no statistical differences（ P 〉 0. 05） and all bone marrows cells were hyperplastic. Conclusion In this study,the rapid initiation and activation of hematopoietic function have been observed which,we believe,is promoted by advanced autologous CRBC apheresis.

关 键 词：浓缩红细胞 造血功能 造血启动速度 造血速度 骨髓造血 动物实验 山羊 

分 类 号：R446.11[医药卫生—诊断学] R331.2[医药卫生—临床医学]