多重耐药鲍曼不动杆菌β-内酰胺类及氨基糖苷类耐药基因检测分析  

Detection and analysis of beta lactam and aminoglycoside resistance genes in multidrug- resistant Acinetobacter baumannii

作　　者：柳晓蕾 孙开宇[2] 贾微 王振明[2] 郭文臣[2] 杨宝宏 

机构地区：[1]潍坊医学院,山东潍坊261000 [2]潍坊市人民医院,山东潍坊261000

出　　处：《医学检验与临床》2017年第10期19-22,共4页Medical Laboratory Science and Clinics

摘　　要：目的：调查我院多重耐药鲍曼不动杆菌（MDR-AB）中β-内酰胺类及氨基糖苷类耐药基因的流行情况，为加强控制临床感染提供依据。方法：采用VITEK-2Compact全自动细菌鉴定仪鉴定菌种，收集2016年10月到2017年3月潍坊市人民医院住院患者感染标本中分离得到的MDR-AB菌株，采用PCR技术检测TEM、ADC、OXA-23、OXA-51、ISAba1、annA、aac（3）-I、ant（3”）-I、aac（6’）-Ib耐药基因的携带情况。结果：TEM、ADC、OXA-23、OXA-51、ISAba1、armA的检出率〉70％，aac（3）-I、ant（3”）-I、aac（6）-Ib检出率〉30％，其中OXA-51、ISAba1全邵检出。结论：我院MDR-AB菌株中TEM、ADC、OXA-23、OXA-51、ISAba1、armA基因携带率高，应加强促进抗生素的合理使用，减缓鲍曼不动杆菌进一步耐药。Objective： To investigate the prevalence of beta lactam and aminoglycoside resistance genes in multidrug-resistant Acinetobacterbaumannii （MDk-AB） of our hospital, providing evidence for clinical control of infection. Methods： Using vitek-2 Compact automatic bacterial identification instrument to identify the bacterial species, MDR-AB strains were isolated from hospitalized patients of Weifang People＇s Hospital during October 2016 to March 2017 , PCR. was used to detect the resistance gene： TEM, ADC, OXA-23, OXA-51, ISAbal, armA, ant （3 ＂） - I,aac （3） - I, aac （6 ＂） -Ib. Results： The detection rate ofTEM, ADC, OXA-23, OXA-51, ISAbal, and armA was 〉70%, and the detection rate ofaac （3） -I, ant （3＂） -I, aac （6＇） -Ib was 〉30%. Besides, the OXA-51 and ISAbal gene were all detected. Conclusion： The carrier rates ofTEM, ADC, OXA-23,OXA-51, ISAbal and armA genes were high in the MDR-AB strain of our hospital.The rational use of antibiotics should be strengthened to slow down the further resistance of the Acinetobacter baumannii.

关 键 词：鲍曼不动杆菌 多重耐药 β-内酰胺类耐药基因 蠹.基糖苷类耐药基因 

分 类 号：R37[医药卫生—病原生物学]