响应面法优化短短芽胞杆菌ch2-22的发酵工艺  

Response Surface Methodology to Optimize Fermentation Technology of Brevibacillus brevis ch2-22

作　　者：孙文 刘倩倩[2,3] 胡彦婷 王雅娜 程辉彩[2,3] 张根伟 张丽萍[2,3] 

机构地区：[1]河北工业大学化工学院,天津300130 [2]河北省科学院生物研究所,河北石家庄050081 [3]河北省主要农作物病害微生物控制工程技术研究中心,河北石家庄050081

出　　处：《微生物学杂志》2017年第6期62-69,共8页Journal of Microbiology

基　　金：河北省科技计划项目(16292904D);河北省科学院重点项目(16302)

摘　　要：在摇瓶发酵条件下,优化提高短短芽胞杆菌ch2-22芽胞浓度和抑菌活性的发酵培养基和培养条件。首先在单因素试验基础上进行响应面设计对ch2-22的发酵培养基进行优化,然后使用单因素试验方法确定最佳发酵条件,得到优化发酵培养基为淀粉35.05 g/L,豆饼粉26.08 g/L,蔗糖10 g/L,鱼粉5 g/L,Na Cl 1 g/L,Mg SO40.3 g/L,(NH4)2SO43 g/L,Mn SO40.1 g/L,K2HPO43 g/L,酵母膏1 g/L,Ca CO32 g/L。培养条件为温度32℃,转速180 r/min,装液量100 m L/500 m L,接种量4%,初始pH为7.0,发酵时间45 h。优化后的芽胞数量达到8.1×109cfu/m L,与优化前的芽胞数量(1.6×109cfu/m L)相比,提高了3.7倍,优化后发酵液效价达到3 350 IU/m L,提高了109%,高于同类菌株的2 000 IU/m L。Fermentation medium and culture conditions to improve the spore concentration and antifungal activity of Brevibacillus brevis ch2-22 at the condition in shaking-flask were optimized. Firstly,based on single factor experiment the ferment medium for B. brevis ch-22 was optimized by response surface methodology,then confirmed the optimized ferment conditions with single factor experiment,obtained the optimized ferment medium was as followed,starch35. 05 g/L,soybean powder 26. 08 g/L,sucrose 10 g/L,fish meal 5 g/L,Na Cl 1 g/L,Mg SO40. 3 g/L,（NH4）2 SO43 g/L,Mn SO40. 1 g/L,K2 HPO43 g/L,yeast extract 1 g/L,Ca CO32 g/L,incubated at 32 ℃,shaking 180,filling at volume 100 mL in 500 mL,inoculum at 4%,initial pH 7. 0,culture for 45 h. The colony-forming units per milliliter were improved from 1. 6 × 109 to 8. 1 × 10^9 after optimization,and the spore concentration was increased by 3. 7 times. The broth valance reached as much as 3 350 IU/mL,increased by 109%,2 000 IU/mL higher than the strain of same kinds.

关 键 词：生物防治 短短芽胞杆菌 发酵优化 响应面法 

分 类 号：Q93-335[生物学—微生物学]