紫外-可见分光光度法测定生大黄和醋大黄的总蒽醌含量  

DETERMINATION OF TOTAL ANTHRAQUINONE CONTENTS IN RAW RHUBARB AND VINEGAR RHUBARB BY ULTRAVIOLET-VISIBLE SPECTROPHOTOMETRY

作　　者：魏江存 陈勇 谢臻 李耀华 邓丽红 阙祖亮 庞丹青 

机构地区：[1]广西中医药大学药学院,广西南宁530020

出　　处：《井冈山大学学报（自然科学版）》2017年第6期88-92,共5页Journal of Jinggangshan University (Natural Science)

基　　金：国家自然科学基金项目(.81360524;81260673);广西壮瑶药协同创新中心(桂教科研[2013]20号);广西壮瑶药重点实验室(桂科基字[2014]32号);广西重点学科壮药学(桂教科研[2013]16号);广西八桂学者中药创新理论与药效研究项目(J13162);广西中医药大学科研创新项目(YJS201625);广西高校中药提取纯化与质量分析重点实验室(桂教科研[2014]6号)

摘　　要：目的建立紫外分光光度法测定生大黄和醋大黄总蒽醌的含量。方法以大黄素为对照品,浓度为1%的醋酸镁甲醇溶液对其进行显色,在513 nm波长处测定吸光度,测定生大黄和醋大黄总蒽醌的含量。结果比较其含量差异,揭示大黄炮制前后总蒽醌的含量变化。结论该方法可作为大黄不同炮制品的质量控制方法。Objective： The method of ultraviolet-visible spectrophotometry for determining the contents of total anthraquinone in raw rhubarb and vinegar rhubarb was established. Methods： Using emodin as the reference substance, 1% magnesium acetate methanol solution for its colration, the absorbance was measured at the wavelength of 513 nm for determing the contents of total anthraquinone in Raw rhubarb. Results： Vinegar rhubarb, and the differences were compared for assure the change of the content of total anthraquinone before and after the processing of rhubarb. Conclusion：This method can be used as a quality control method for different processed products of rhubarb.

关 键 词：生大黄 醋大黄 总蒽醌 紫外分光光度法 

分 类 号：R284.2[医药卫生—中药学]