油茶叶绿素提取方法的比较研究  

Comparison of Chlorophyll Extraction Methods in Camellia oleifera

作　　者：程贵文 龚洪恩[1] 颜送宝 陈传松[1] 罗嘉东 袁小平[1] 吴建国[1] 

机构地区：[1]中国林业科学研究院亚热带林业实验中心,新余336600

出　　处：《湖北林业科技》2017年第6期11-13,58,共4页Hubei Forestry Science and Technology

基　　金：公益性行业科研专项经费项目"油茶高产高效栽培气象保障关键技术研究"(GYHY201506017);中国林科院亚热带林业实验中心基金项目"油茶优良杂交子代收集及性状测定"(083)

摘　　要：为寻求油茶叶绿素提取效果最好的方法,以长林4号油茶叶片为试材,研究5种不同提取方法对油茶叶片叶绿素提取效果的影响。结果表明:不同提取方法提取效果差异显著,提取效果最好的是丙酮∶乙醇=2∶1的混合液,其次是丙酮∶乙醇=1∶1的混合液,然后是丙酮∶乙醇∶水=4.5∶4.5∶1的混合液,80%丙酮和95%乙醇提取效果相对较差。丙酮∶乙醇2∶1混合液法比较适合油茶叶绿素的提取。In order to find out the best extraction method of chlorophyll in Camellia oleifera,the effects of five different extraction methods on the extraction of chlorophyll were studied,with Camellia oleifera leaves of Changlin 4 as experimental material.The results showed that different extraction methods showed significant differencethe,mixed reagent of acetone and ethyl alcohol in ratio of 2∶1 had the best effect,followed by the mixed reagent of acetone and ethyl alcohol in ratio of 1∶1,then the mixed reagent of acetone,ethyl alcohol and water in ratio of 4.5∶4.5∶1,80% acetone and95% ethanol had relatively poor effect.The mixture of acetone and ethanol in ratio of 2：1 is suitable for the extraction of chlorophyll from Camellia oleifera.

关 键 词：油茶 叶绿素 提取方法 

分 类 号：S722.3[农业科学—林木遗传育种]