新生儿外周血有核红细胞检测的临床应用  

Clinical application of nucleated red blood cells detection in peripheral blood of neonates

作　　者：陈雪瑛 陈洪平[1] 龚娇[1] 周文营[1] 李锦城[1] 胡波[1] 

机构地区：[1]中山大学附属第三医院检验科,广东广州510630

出　　处：《中国卫生检验杂志》2018年第2期215-216,219,共3页Chinese Journal of Health Laboratory Technology

摘　　要：目的探讨新生儿外周血有核红细胞(NRBC)检测的临床应用。方法回顾性收集中山大学附属第三医院2016年1月-10月住院的430例新生儿患者为观察组,另随机抽取40例健康足月新生儿为对照组,利用Sysmex XE-5000血液分析仪测定患儿外周血NRBC的比例、计数绝对值,大于正常参考值0%~1%的结果为阳性。结果 430例新生儿患者NRBC总检出率为58.37%,男性组检出率为56.58%(129/228),女性组检出率为60.39%(122/202),两者差异无统计学意义(P>0.05)。早产儿外周血NRBC检出率为99.23%;窒息患儿外周血NRBC检出率为94.44%;产前宫内窘迫婴儿外周血NRBC检出率为93.75%;酸血症患儿外周血NRBC检出率为91.67%;高胆红素血症患儿外周血NRBC检出率为3.41%。各类型患儿检出率之间比较,差异有统计学意义(P<0.05)。结论早产儿、新生窒息胎儿、产前宫内窘迫婴儿、酸血症患儿外周血NRBC计数比例与绝对值均会升高,高胆红素血症患儿外周血NRBC检出率较低,测定外周血NRBC可用于评价新生儿健康状态。Objective To explore the clinical application of nucleated red blood cells（ NRBC） in peripheral blood of neonates.Methods We retrospectively collected 430 newborns hospitalized in the Third Affiliated Hospital of Sun Yat-sen University from January to October,2016,and 40 healthy full-term newborns were randomly selected as the control group. The NRBC ratio and the absolute value of count in peripheral blood were measured by Sysmex XE-5000 hematology analyzer,regarding the results greater than the normal reference of 0%-1% as positive. Results The detection rate of NRBC in 430 neonates was58. 37%,the detection rate was 56. 58%（ 129/228） in male and 60. 39%（ 122/202） in female,with the difference between both of them not statistically significant（ P〉 0. 05）. The detection rate of NRBC in peripheral blood of prematurity was99. 23%; the detection rate of NRBC in peripheral blood of asphyxia children was 94. 44%; the detection rate of NRBC in peripheral blood of prenatal uterine distress infants was 93. 75%; the detection rate of NRBC was 91. 67%; the detection rate of NRBC in children with hyperbilirubinemia was 3. 41%. The detection rate differences of types of children were statistically significant（ P 〈0. 05）. Conclusion Premature infants,neonatal asphyxia,antenatal fetal intrauterine distress,newborn babies acidemia children with peripheral blood NRBC count percentage and absolute value will increase. The detection rate of peripheral blood NRBC in neonates with neonatal hyperbilirubinemia is low. The measurement of peripheral blood NRBC percentage and absolute value helps to evaluate neonatal＇s health status.

关 键 词：新生儿 有核红细胞 早产儿 新生儿窒息 产前宫内窘迫婴儿 酸血症患儿 高胆红素血症 

分 类 号：R722.12[医药卫生—儿科]