多效唑对桃枝枯病的调控作用研究  

Effect of paclobutrazol on control of peach shoot blight

作　　者：纪兆林[1] 汪孝璊 王汝艳 董京萍[1] 徐敬友[1] 童蕴慧[1] 金唯新 宋宏峰[3] 

机构地区：[1]扬州大学园艺与植物保护学院,江苏扬州225009 [2]无锡市阳山镇农业服务中心,江苏无锡214155 [3]江苏省农业科学院果树研究所,江苏南京210014

出　　处：《扬州大学学报（农业与生命科学版）》2017年第4期99-104,共6页Journal of Yangzhou University：Agricultural and Life Science Edition

基　　金：国家现代农业产业技术体系建设专项(CARS-30-3-02);江苏省农业科技自主创新资金项目[CX(14)2015;CX(15)1020];无锡市农业科技支撑计划项目(CLE01N1410)

摘　　要：为明确植物生长调节剂多效唑对桃枝枯病的调控作用,通过毒力测定、离体枝条接种、H_2O_2和丙二醛(MDA)含量测定,研究多效唑对桃枝枯病菌的抑制作用及对病菌侵染和病害的控制作用。结果表明:多效唑对桃枝枯病菌的EC50值为1.623 mg·L^(-1),能显著破坏菌丝形态,使菌丝畸形、细胞膨大;400、500、600、700及800 mg·L^(-1)多效唑药液均能在一定程度上影响枝枯病菌在桃树离体枝条上的扩展,其中600 mg·L^(-1)多效唑抑制作用最为明显,病斑大小较清水对照和甲醇对照分别减少71.15%和69.70%。600 mg·L^(-1)多效唑对病菌侵染后枝条H_2O_2和MDA含量均有明显的降低作用。因此,一定浓度的多效唑能减轻病菌对寄主的危害,对病菌侵染的抑制作用可能与通过提高抗氧化物质活性和调控寄主抗病性有关。The plant growth regulator paclobutrazol is widely used in agriculture to inhibit plant growth,promote tillering,increase plant yield and so on.In order to provide the basis for control peach shoot blight by paclobutrazol,this paper used the pathogen of peach shoot blight,Phomopsis amygdali,as test strain,and measured the peach shoot blight lesion size and the content of H2O2 and MDA in the shoots after spraying the paclobutrazol,using shoot inoculation in vitro.The results showed that paclobutrazol had the virulence to P.amygdali,with EC50 value of 1.623 mg·L-（-1）,and had a destructive effect on hyphal shape of P.amygdali,with hyphal cell distortion and tumescence.The 400,500,600,700 and 800 mg·L-（-1） paclobutrazol could control extension of disease spot on the twigs in vitro in different degrees.Among them,the inhibitory effect of the concentration of 600 mg·L-（-1） for paclobutrazol was the best,and the lesion size was reduced by 71.15% and 69.70%,respectively,compared with the water control and methanol control.The 600 mg·L-（-1） paclobutrazol had a significant effect on reducing content of H2O2 and MDA in P.amygdali infected twigs.Therefore,a certain concentration of paclobutrazol could reduce the pathogen damage to the host,and the inhibitory effect of paclobutrazol on pathogen infection may be related to the regulation of host disease resistance by increasing the activity of antioxidant substances.

关 键 词：桃 桃枝枯病 桃拟茎点霉 多效唑 抗病性 

分 类 号：S436[农业科学—农业昆虫与害虫防治] S662.1[农业科学—植物保护]