负压创面影响肉芽血管生成过程中miR-195和NLRX1表达研究  

The negative-pressure wound therapy promotes wound healing by enhancing angiogenesis in granulation tissue and wound healing through suppression of NLRX1 through via the up-regulation of miR-195expression by suppressing NLRX1 expression upregulation

作　　者：陈晓霞 唐宁宁 谢慧清[1] 钱利[3] 胡丰[1] 王楚望 贺斌 徐家钦[5] 梁尊鸿[5] 周建大[1] 

机构地区：[1]中南大学湘雅三医院整形外科,长沙410013 [2]湘雅常德医院整形外科,湖南省常德415001 [3]中南大学湘雅二医院烧伤整形科,长沙410011 [4]湖南省宁乡市人民医院整形外科,410602 [5]海南省人民医院整形科,海口570311

出　　处：《中国医师杂志》2018年第2期183-186,190,共5页Journal of Chinese Physician

基　　金：湖南省自然科学基金(2015JJ4053);湖南省卫计委科研项目(B2016-126;B2015-40)~~

摘　　要：目的探讨miR-195及NLRX1在负压治疗后创面肉芽组织中的表达及其与创面血管生成的关系。方法收集6例经创面负压治疗前后的难愈性创面患者肉芽组织,检测其组织中miR-195、NLRX1 mRNA表达水平,NLRX1蛋白表达水平;同时进行免疫组化(IHC)检测其组织中NLRX1的表达及CD31标记的组织微血管密度(MVD)。结果在负压治疗后7 d的肉芽组织中miR-195、MVD均显著高于治疗前(P<0.05),而NLRX1则明显低于治疗前(P<0.05);肉芽组织中miR-195与NLRX1的表达呈负相关(r=-0.856,P<0.001);肉芽组织中NLRX1的表达与MVD呈负相关(r=-0.618,P<0.05);肉芽组织中miR-195的表达与MVD呈正相关(r=0.630,P<0.05)。结论负压创面治疗能促进肉芽血管生成,促进创面愈合;负压创面治疗可能通过调节miR-195的表达上调,抑制NLRX1的表达促进血管生成。Objective To investigate the expression and the relationship with angiogenesis of miR- 195 and NLR family member X1 （ NLRX1 ） in granulation tissue after negative-pressure wound treatment （NPWT）. Methods Six patients were collected who received negative pressure treatment with refractory wound granulation. The levels of miR-195, NLRX1 mRNA and NLRX1 proteins were measured. The expression of NLRX1 and the micro-vascular density （MVD） of CD31 were detected by immunohistochemistry （IHC）. Results MiR-195 and MVD were significantly higher in granulation tissue after 7 days negative pressure treatment （ P 〈 0. 05 ）, and NLRX1 was significantly lower （ P 〈 0. 05 ）. In granulation tissue, the expression of miR-195 was negatively correlated with NLRX1 （ r = - 0. 856, P 〈 0. 001 ） , the expression of NLRXI was negatively correlated with MVD （r = -0. 618, P 〈0. 05）, and the expression of miR-195 was positively correlated with MVD （ r = 0. 630, P 〈 0. 05 ）. Conclusions Negative pressure wound therapy can promote the formation of granulation vessels and the wound healing. The therapeutic mechanism may in-hibit the expression of NLRX1 and upregulate the expression of miR-195 to promote angiogenesis.

关 键 词：负压伤口疗法 微RNAs/代谢 NOD样受体/代谢 肉芽组织/血液供给 肉芽组 织/病理学 新生血管化 病理性 

分 类 号：R641[医药卫生—外科学]