健骨冲剂对大鼠膝骨性关节炎模型关节软骨中AQP3表达的影响及意义  

Effect of Jiangu granule on the expression of AQP3 in articular cartilage of rats with knee osteoarthritis

作　　者：吴兆黎 

机构地区：[1]湖南省人民医院(湖南师范大学附属第一医院)中西医结合科,长沙410016

出　　处：《中国医师杂志》2018年第2期200-203,共4页Journal of Chinese Physician

基　　金：湖南省中医药科研计划项目(2017105)~~

摘　　要：目的探讨健骨冲剂对大鼠膝骨性关节炎(OA)关节软骨中水通道蛋白3(AQP3)表达的影响及意义。方法 SD大鼠随机分为假手术组、模型组、健骨冲剂治疗组,其中治疗组分为健骨冲剂高剂量、中剂量和低剂量3亚组,每组12只。利用膝关节注射4%木瓜蛋白酶与0.03 mol/L L-半胱氨酸混合液的方法制作骨关节炎模型。假手术组和模型组每日正常喂养,治疗组(高、中、低剂量组)造模完成后第2天开始,分别给予不同剂量健骨冲剂灌胃给药,2次/d,连续4周。治疗完统一处死实验大鼠,取膝关节软骨,采用大体光镜和组织学病理切片观察关节软骨破坏情况;RealtimePCR法和Western blot法检测各组大鼠右膝关节软骨内AQP3的表达变化。结果健骨冲剂治疗组的关节软骨破坏程度明显轻于模型组(P<0.05);模型组AQP3的mRNA和蛋白表达水平明显高于假手术组(P<0.05);OA大鼠用健骨冲剂治疗4周后,关节软骨中AQP3的mRNA和蛋白表达水平显著下降,健骨冲剂各亚组与模型组比较差异均有统计学意义(P<0.05),其中高剂量组下降最为明显。结论健骨冲剂能降低大鼠膝OA关节软骨中AQP3的表达,从而缓解关节肿胀,可能具有关节软骨保护作用。ObjectiveTo investigate the effect and significance of Jiangu granule on the expression of aquaporin 3 （AQP3） in articular cartilage of knee osteoarthritis （OA） rats. MethodsSprague-Dawley （SD） rats were randomly divided into sham operation group, model group, and Jiangu granule treatment group. The treatment group was divided into Jiangu granule high dose, medium dose, and low dose groups, with 12 rats in each group. The osteoarthritis model was made by injecting 4% papain and 0.03 mol/L L-cysteine mixture into the knee joint. Sham-operated group and model group received normal feeding every day. Treatment group （high, medium, and low dose groups）, the next day after the completion of the model, were given different dose Jiangu granule by gavage twice a day for 4 weeks. After treatment, the rats were executed uniformly, using general optical microscope and the pathological section of histology to observe the articular cartilage damage. The expression of AQP3 in articular cartilage of rats was detected by real time polymerase chain reaction （RT-PCR） and Western blot. ResultsThe articular cartilage destruction of Jiangu granule treatment group was significantly less than that of the model group （P〈0.05）. The mRNA and protein expression levels of AQP3 in model group was significantly higher than that in the sham operation group （P〈0.05）. OA rats with Jiangu granule after 4 weeks treatment, the mRNA and protein expression levels of AQP3 in articular cartilage were decreased significantly. Jiangu granule in each sub group relative to the model group had statistical significance （P〈0.05）, and the high dose group was decreased significantly. ConclusionsJiangu granule can reduce the expressions of AQP3 in OA articular cartilage of rats and alleviate the swelling of joints, which may have protective effects on articular cartilage.

关 键 词：骨关节炎 膝/中药疗法 中草药/投药和剂量 软骨 关节/病理学/代谢 水通道蛋白质3/代谢 大鼠 

分 类 号：R285.5[医药卫生—中药学]